biostudies-otherUnknownJeroen PenningsHorizon Europe Research and Innovation ProgrammeEuropean Union’s Horizon 2020 Research and Innovation ProgrammeHomo sapiens (human)https://www.ebi.ac.uk/biostudies/studies/S-BSST2296biostudies-other101057014964537Jeroen PenningsfalseMalondialdehyde formation in HepG2 cells after compound exposureWe quantified malondialdehyde formation in maturated HepG2 cells after 24 h of compound exposure. This was done using three exposure scenarios: (1) cells receiving acute exposure; (2) cells receiving repeated exposure; (3) cells receiving repeated exposure and recovery. Lipid peroxidation was assessed by measuring malondialdehyde (MDA) levels using the thiobarbituric acid reactive substances (TBARS) assay [Wong et al. Clin Chem. 1987;33(2 Pt1):214-20]. Fluorescence was measured in the supernatant at λexc= 530 nm and λem= 575 nm using a 96-wells fluorescence plate reader. The MDA levels were quantified using a standard curve. This study was performed as part of the RISK-HUNT3R project and the PARC project.2026-03-31T00:00:00Z2026-06-29T04:01:37.936Z2025-11-20T12:23:14.493ZS-BSST2296