{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Boulaire Jerome"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-12305"],"project":["EurocanPlatform"],"abstract":["Achievement of specific tumor cell targeting remains a challenge for glioma gene therapy. We report here the identification and characterization of a 5’ sequence of human HMGB2 gene for transcriptional targeting to glioblastoma. We performed microarray analysis and found HMGB2 as one of the genes that had a low level of expression in normal human astrocytes, but was significantly up-regulated in glioblastoma cells. Real-time PCR quantification revealed increase in HMBG2 expression level in glioblastoma tissues and cells between 11 to 79 fold over that in normal human brain tissue. With progressive truncation of a 5’-upstream sequence of the HMGB2 gene, we identified a 500-bp fragment that displayed a high transcriptional activity in glioblastoma cells, but a low activity in normal brain ce"],"repository":["biostudies-other"],"experiment_type":["transcription profiling by array"],"data_source":["EurocanPlatform"],"pubmed_authors":["Wang Shu","Zhao Ying","Lin Jiakai","Boulaire Jerome","Zeng Jieming","Balani Poonam"],"additional_accession":[]},"is_claimable":false,"name":"A 5? Sequence of Human HMGB2 Gene for Transcriptional Targeting of Glioblastoma","description":"Achievement of specific tumor cell targeting remains a challenge for glioma gene therapy. We report here the identification and characterization of a 5’ sequence of human HMGB2 gene for transcriptional targeting to glioblastoma. We performed microarray analysis and found HMGB2 as one of the genes that had a low level of expression in normal human astrocytes, but was significantly up-regulated in glioblastoma cells. Real-time PCR quantification revealed increase in HMBG2 expression level in glioblastoma tissues and cells between 11 to 79 fold over that in normal human brain tissue. With progressive truncation of a 5’-upstream sequence of the HMGB2 gene, we identified a 500-bp fragment that displayed a high transcriptional activity in glioblastoma cells, but a low activity in normal brain ce","dates":{"release":"2016-04-14T13:29:35Z","modification":"2016-04-14T13:29:35Z","creation":"2016-04-14T13:29:35Z"},"accession":"S-ECPF-GEOD-12305","cross_references":{"GEO":["GSE12305"],"ArrayExpress":["E-GEOD-12305"],"EFO":["EFO_0000322","EFO_0000519"],"ArrayExpress files":["E-GEOD-12305"]}}