{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Darvishian Farbod"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-24824"],"project":["EurocanPlatform"],"abstract":["To identify genes differentially modulated by anti-miR-182 treatment in a liver melanoma metastasis mouse model. Targeting oncogenic microRNAs is emerging as a promising strategy for cancer therapy. Here we provide proof-of-principle for the safety and efficacy of miRNA targeting against metastatic tumors. We tested the effect of targeting miR-182, a pro-metastatic miRNA frequently overexpressed in melanoma, whose silencing represses invasion and induces apoptosis in vitro. In particular, we assessed the effect of anti-miR-182 oligonucleotides synthesized with 2’ sugar modifications and a phosphorothioate backbone in a mouse model of melanoma liver metastasis. Luciferase imaging showed that mice treated with anti-miR-182 had an appreciably lower burden of liver metastases compared to the c"],"repository":["biostudies-other"],"experiment_type":["transcription profiling by array"],"data_source":["EurocanPlatform"],"pubmed_authors":["Levin Brandi","Segura Miguel","Osman Iman","Menendez Silvia","Zavadil Jiri","Darvishian Farbod","Huynh Chanh","Meruelo Daniel","Hernando Eva","Gaziel Avita","Chiriboga Luis"],"additional_accession":[]},"is_claimable":false,"name":"Identification of genes and molecular pathways associated with anti-miR-182 treatment","description":"To identify genes differentially modulated by anti-miR-182 treatment in a liver melanoma metastasis mouse model. Targeting oncogenic microRNAs is emerging as a promising strategy for cancer therapy. Here we provide proof-of-principle for the safety and efficacy of miRNA targeting against metastatic tumors. We tested the effect of targeting miR-182, a pro-metastatic miRNA frequently overexpressed in melanoma, whose silencing represses invasion and induces apoptosis in vitro. In particular, we assessed the effect of anti-miR-182 oligonucleotides synthesized with 2’ sugar modifications and a phosphorothioate backbone in a mouse model of melanoma liver metastasis. Luciferase imaging showed that mice treated with anti-miR-182 had an appreciably lower burden of liver metastases compared to the c","dates":{"release":"2016-04-14T13:48:51Z","modification":"2016-04-14T13:48:51Z","creation":"2016-04-14T13:48:51Z"},"accession":"S-ECPF-GEOD-24824","cross_references":{"GEO":["GSE24824"],"ArrayExpress":["E-GEOD-24824"],"EFO":["EFO_0000756","EFO_0003942"],"ArrayExpress files":["E-GEOD-24824"]}}