biostudies-otherUnknownDarvishian FarbodHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-24824EurocanPlatformTo identify genes differentially modulated by anti-miR-182 treatment in a liver melanoma metastasis mouse model. Targeting oncogenic microRNAs is emerging as a promising strategy for cancer therapy. Here we provide proof-of-principle for the safety and efficacy of miRNA targeting against metastatic tumors. We tested the effect of targeting miR-182, a pro-metastatic miRNA frequently overexpressed in melanoma, whose silencing represses invasion and induces apoptosis in vitro. In particular, we assessed the effect of anti-miR-182 oligonucleotides synthesized with 2’ sugar modifications and a phosphorothioate backbone in a mouse model of melanoma liver metastasis. Luciferase imaging showed that mice treated with anti-miR-182 had an appreciably lower burden of liver metastases compared to the cbiostudies-othertranscription profiling by arrayEurocanPlatformLevin BrandiSegura MiguelOsman ImanMenendez SilviaZavadil JiriDarvishian FarbodHuynh ChanhMeruelo DanielHernando EvaGaziel AvitaChiriboga LuisfalseIdentification of genes and molecular pathways associated with anti-miR-182 treatmentTo identify genes differentially modulated by anti-miR-182 treatment in a liver melanoma metastasis mouse model. Targeting oncogenic microRNAs is emerging as a promising strategy for cancer therapy. Here we provide proof-of-principle for the safety and efficacy of miRNA targeting against metastatic tumors. We tested the effect of targeting miR-182, a pro-metastatic miRNA frequently overexpressed in melanoma, whose silencing represses invasion and induces apoptosis in vitro. In particular, we assessed the effect of anti-miR-182 oligonucleotides synthesized with 2’ sugar modifications and a phosphorothioate backbone in a mouse model of melanoma liver metastasis. Luciferase imaging showed that mice treated with anti-miR-182 had an appreciably lower burden of liver metastases compared to the c2016-04-14T13:48:51Z2016-04-14T13:48:51Z2016-04-14T13:48:51ZS-ECPF-GEOD-24824GSE24824E-GEOD-24824EFO_0000756EFO_0003942E-GEOD-24824