{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"submitter":["Novellasdemunt L"],"pagination":["345-356"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-25077"],"project":["EurocanPlatform"],"abstract":["Breast cancer cell lines containing the progesterone receptor respond to progestins, altering expression of a subset of genes. In a previously published experiment of inducible knock-down of histone H1 isoforms with an shRNA-expression lentivector  (GSE12299), we modified the breast cancer cell line T47D with different vectors. Here, we explored response to progestin R5020 of control cells generated with the empty shRNA-expression vector. Stable breast cancer-derived cell lines containing the empty vector for inducible shRNA expression were grown for two days in the absence of serum. Progestin R5020 or vehicle was added for 6 hours, in duplicate, and RNA was extracted for microarray hybridization."],"repository":["biostudies-other"],"experiment_type":["transcription profiling by array"],"data_source":["EurocanPlatform"],"omics_type":["Unknown"],"volume":["442"],"journal":["The Biochemical journal"],"species":["Homo sapiens"],"pubmed_authors":["Novellasdemunt L","Millán-Ariño L","Navarro-Sabate A","Jordan A","Bartrons R","Obach M","Ventura F","Manzano A","Rosa JL"],"additional_accession":[]},"is_claimable":false,"name":"Gene expression in response to progestin R5020","description":"Breast cancer cell lines containing the progesterone receptor respond to progestins, altering expression of a subset of genes. In a previously published experiment of inducible knock-down of histone H1 isoforms with an shRNA-expression lentivector  (GSE12299), we modified the breast cancer cell line T47D with different vectors. Here, we explored response to progestin R5020 of control cells generated with the empty shRNA-expression vector. Stable breast cancer-derived cell lines containing the empty vector for inducible shRNA expression were grown for two days in the absence of serum. Progestin R5020 or vehicle was added for 6 hours, in duplicate, and RNA was extracted for microarray hybridization.","dates":{"release":"2016-04-14T13:49:18Z","publication":"2012 Mar","modification":"2016-04-14T13:49:18Z","creation":"2016-04-14T13:49:18Z"},"accession":"S-ECPF-GEOD-25077","cross_references":{"GEO":["GSE25077"],"ArrayExpress":["E-GEOD-25077"],"EFO":["EFO_0000305","EFO_0000322"],"ArrayExpress files":["E-GEOD-25077"]}}