{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"submitter":["Prenzel T"],"pagination":["5739-5753"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-31118"],"project":["EurocanPlatform"],"abstract":["The estrogen receptor-alpha (ERα) determines breast cancer cell phenotype and is a prognostic indicator. A better understanding of the mechanisms controlling ERα function may uncover improved strategies for the treatment of breast cancer. Proteasome inhibition was previously reported to regulate estrogen-induced transcription but the mechanisms by which it influences ERα function remain controversial. In this study we investigated the transcriptome-wide effects of the proteasome inhibitor Velcade on estrogen-regulated transcription in MCF7 human breast cancer cells and demonstrate a specific global decrease in estrogen-induced transcription. This set contains 21 microarray samples. 3 controls, 3 estrogen stimulated, 3 Bortezomib + estrogen stimulated, 2* 3 siRNA controls, 3 siRNA PSMB3 knockdowns, 3 siRNA PSMB5 knockdowns"],"repository":["biostudies-other"],"experiment_type":["transcription profiling by array"],"data_source":["EurocanPlatform"],"omics_type":["Unknown"],"volume":["71"],"journal":["Cancer research"],"species":["Homo sapiens"],"pubmed_authors":["Johnsen SA","Begus-Nahrmann Y","Hsu C","Kramer F","Prenzel T","Eick D","Beissbarth T","Simons M","Gorsler T","Kremmer E","Hennion M","Hintermair C"],"additional_accession":[]},"is_claimable":false,"name":"Estrogen-dependent gene expression in human breast cancer cells relies upon proteosome-dependent monoubiquitination of histone H2B","description":"The estrogen receptor-alpha (ERα) determines breast cancer cell phenotype and is a prognostic indicator. A better understanding of the mechanisms controlling ERα function may uncover improved strategies for the treatment of breast cancer. Proteasome inhibition was previously reported to regulate estrogen-induced transcription but the mechanisms by which it influences ERα function remain controversial. In this study we investigated the transcriptome-wide effects of the proteasome inhibitor Velcade on estrogen-regulated transcription in MCF7 human breast cancer cells and demonstrate a specific global decrease in estrogen-induced transcription. This set contains 21 microarray samples. 3 controls, 3 estrogen stimulated, 3 Bortezomib + estrogen stimulated, 2* 3 siRNA controls, 3 siRNA PSMB3 knockdowns, 3 siRNA PSMB5 knockdowns","dates":{"release":"2016-04-14T13:59:58Z","publication":"2011 Sep","modification":"2016-04-14T13:59:58Z","creation":"2016-04-14T13:59:58Z"},"accession":"S-ECPF-GEOD-31118","cross_references":{"GEO":["GSE31118"],"ArrayExpress":["E-GEOD-31118"],"EFO":["EFO_0000305","EFO_0000322"],"ArrayExpress files":["E-GEOD-31118"]}}