biostudies-otherPang B1908https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-33624EurocanPlatformOne major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions. As a result, the epigenome and the transcriptome are strongly affected. Tissue culture cells were treated with doxorubicin, aclarubicin or etoposide for 2 hours. Then drugs were removed by extensive washing. cells were further cultured for indicated days before total RNA were extracted and compared to un-treated contrbiostudies-othertranscription profiling by arrayEurocanPlatformUnknown4Nature communicationsHomo sapiensPang Bvan Tellingen OJanssen JJanssen LHuijgens PKerkhoven RGroothuis TRottenberg SZwart WQiao XNeefjes JOvaa HNieuwland MVelds AfalseDistinct effects of topoisomerase II inhibitors on tumor cell lines (part 1)One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions. As a result, the epigenome and the transcriptome are strongly affected. Tissue culture cells were treated with doxorubicin, aclarubicin or etoposide for 2 hours. Then drugs were removed by extensive washing. cells were further cultured for indicated days before total RNA were extracted and compared to un-treated contr2016-04-14T14:05:17Z20132016-04-14T14:05:17Z2016-04-14T14:05:17ZS-ECPF-GEOD-33624GSE33624E-GEOD-33624EFO_0000756EFO_0000365EFO_0000322E-GEOD-33624