{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"submitter":["Pang B"],"pagination":["1908"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-33625"],"project":["EurocanPlatform"],"abstract":["One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells.  Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions. As a result, the epigenome and the transcriptome are strongly affected. Tissue culture cells were treated with doxorubicin, aclarubicin or etoposide for 2 hours. Then drugs were removed by extensive washing. cells were further cultured for indicated days before total RNA were extracted and compared to un-treated contr"],"repository":["biostudies-other"],"experiment_type":["transcription profiling by array"],"data_source":["EurocanPlatform"],"omics_type":["Unknown"],"volume":["4"],"journal":["Nature communications"],"species":["Homo sapiens"],"pubmed_authors":["Pang B","van Tellingen O","Janssen J","Janssen L","Huijgens P","Kerkhoven R","Groothuis T","Rottenberg S","Zwart W","Qiao X","Neefjes J","Ovaa H","Nieuwland M","Velds A"],"additional_accession":[]},"is_claimable":false,"name":"Distinct effects of  topoisomerase II inhibitors on tumor cell lines (part 2)","description":"One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells.  Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions. As a result, the epigenome and the transcriptome are strongly affected. Tissue culture cells were treated with doxorubicin, aclarubicin or etoposide for 2 hours. Then drugs were removed by extensive washing. cells were further cultured for indicated days before total RNA were extracted and compared to un-treated contr","dates":{"release":"2016-04-14T14:05:18Z","publication":"2013","modification":"2016-04-14T14:05:18Z","creation":"2016-04-14T14:05:18Z"},"accession":"S-ECPF-GEOD-33625","cross_references":{"GEO":["GSE33625"],"ArrayExpress":["E-GEOD-33625"],"EFO":["EFO_0000756","EFO_0000365","EFO_0000322"],"ArrayExpress files":["E-GEOD-33625"]}}