biostudies-otherUnknownDenvir JamesHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-36081EurocanPlatformGrainyhead genes are involved in wound healing and developmental neural tube closure. In light of the high degree of similarity between the epithelial-mesenchymal transitions (EMTs) occurring in wound healing processes and the cancer stem cell-like compartment of tumors, including TGF-β-dependence, we investigated the role of a Grainyhead gene (GRHL2) in oncogenic EMT. Grainyhead was specifically down-regulated in the claudin-low subclass of mammary tumors and in the basal-B subclass of breast cancer cell lines. Functionally, GRHL2 suppressed TGF-β-induced, Twist-induced or spontaneous EMT, enhanced anoikis-sensitivity, and suppressed mammosphere generation in mammary epithelial cells. These effects were mediated, in part, by its suppression of ZEB1 expression, through direct repressiobiostudies-othertranscription profiling by arrayEurocanPlatformIvanov AlexeyWidmeyer JosephDenvir JamesFrisch StevenPifer PhillipCieply BenjaminRiley PhilipAddison JosephfalseEffect of GRHL2 in HMLE+Twist-ER+4-OHT cellsGrainyhead genes are involved in wound healing and developmental neural tube closure. In light of the high degree of similarity between the epithelial-mesenchymal transitions (EMTs) occurring in wound healing processes and the cancer stem cell-like compartment of tumors, including TGF-β-dependence, we investigated the role of a Grainyhead gene (GRHL2) in oncogenic EMT. Grainyhead was specifically down-regulated in the claudin-low subclass of mammary tumors and in the basal-B subclass of breast cancer cell lines. Functionally, GRHL2 suppressed TGF-β-induced, Twist-induced or spontaneous EMT, enhanced anoikis-sensitivity, and suppressed mammosphere generation in mammary epithelial cells. These effects were mediated, in part, by its suppression of ZEB1 expression, through direct repressio2016-04-14T14:08:00Z2016-04-14T14:08:00Z2016-04-14T14:08:00ZS-ECPF-GEOD-36081GSE36081E-GEOD-36081EFO_0000305EFO_0000322E-GEOD-36081