{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"submitter":["Dowhan DH"],"pagination":["509-526"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-36542"],"project":["EurocanPlatform"],"abstract":["Protein arginine methyltransferase-6 (PRMT6) regulates steroid-dependent transcription and alternative splicing, and is implicated in endocrine system development and function, cell death, cell cycle, gene expression and cancer. Despite its role in these processes, little is known about its function and cellular targets in breast cancer. To identify novel gene targets regulated by PRMT6 in breast cancer cells, we used a combination of small interfering RNA (siRNA) and exon-specific microarray profiling in vitro, coupled to in vivo validation in normal breast and primary human breast tumours. This approach, which allows the examination of genome-wide changes in individual exon usage and total transcript levels, demonstrated PRMT6 knockdown significantly affected: (i) the transcription of 15"],"repository":["biostudies-other"],"experiment_type":["transcription profiling by array"],"data_source":["EurocanPlatform"],"omics_type":["Unknown"],"volume":["19"],"journal":["Endocrine-related cancer"],"species":["Homo sapiens"],"pubmed_authors":["Brown MA","Harrison MJ","Leedman PJ","Eriksson NA","Pearen MA","Tilley WD","Clarke CL","Dowhan DH","Funder JW","Fuller PJ","Simpson ER","Muscat GE","Bailey P"],"additional_accession":[]},"is_claimable":false,"name":"Protein Arginine Methyltransferase 6 dependent gene expression and splicing: Association with breast cancer outcomes","description":"Protein arginine methyltransferase-6 (PRMT6) regulates steroid-dependent transcription and alternative splicing, and is implicated in endocrine system development and function, cell death, cell cycle, gene expression and cancer. Despite its role in these processes, little is known about its function and cellular targets in breast cancer. To identify novel gene targets regulated by PRMT6 in breast cancer cells, we used a combination of small interfering RNA (siRNA) and exon-specific microarray profiling in vitro, coupled to in vivo validation in normal breast and primary human breast tumours. This approach, which allows the examination of genome-wide changes in individual exon usage and total transcript levels, demonstrated PRMT6 knockdown significantly affected: (i) the transcription of 15","dates":{"release":"2016-04-14T14:08:33Z","publication":"2012 Aug","modification":"2016-04-14T14:08:33Z","creation":"2016-04-14T14:08:33Z"},"accession":"S-ECPF-GEOD-36542","cross_references":{"GEO":["GSE36542"],"ArrayExpress":["E-GEOD-36542"],"EFO":["EFO_0000635","EFO_0000305","EFO_0000322"],"ArrayExpress files":["E-GEOD-36542"]}}