biostudies-otherPersson H78-86https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-39162EurocanPlatformTo comprehensively characterize microRNA (miRNA) expression in breast cancer, we performed the first extensive next-generation sequencing expression analysis of this disease. We sequenced small RNA from tumors with paired samples of normal and tumor-adjacent breast tissue. Our results indicate that tumor identity is achieved mainly by variation in the expression levels of a common set of miRNAs rather than by tissue-specific expression. We also report 361 new, well-supported miRNA precursors. Nearly two-thirds of these new genes were detected in other human tissues and 49% of the miRNAs were found associated with Ago2 in MCF7 cells. Ten percent of the new miRNAs are located in regions with high-level genomic amplifications in breast cancer. A new miRNA is encoded within the ERBB2/Her2 genebiostudies-otherRNA-seq of non coding RNAEurocanPlatformUnknown71Cancer researchHomo sapiensPersson HJonsson GBorg ALuts LRego NStaaf JLoman NNaya HKvist AVallon-Christersson JRovira CHoglund MfalseIdentification of new microRNAs in paired normal and tumor breast tissue suggests a dual role for the ERBB2/Her2 geneTo comprehensively characterize microRNA (miRNA) expression in breast cancer, we performed the first extensive next-generation sequencing expression analysis of this disease. We sequenced small RNA from tumors with paired samples of normal and tumor-adjacent breast tissue. Our results indicate that tumor identity is achieved mainly by variation in the expression levels of a common set of miRNAs rather than by tissue-specific expression. We also report 361 new, well-supported miRNA precursors. Nearly two-thirds of these new genes were detected in other human tissues and 49% of the miRNAs were found associated with Ago2 in MCF7 cells. Ten percent of the new miRNAs are located in regions with high-level genomic amplifications in breast cancer. A new miRNA is encoded within the ERBB2/Her2 gene2016-04-14T14:12:44Z2011 Jan2016-04-14T14:12:44Z2016-04-14T14:12:44ZS-ECPF-GEOD-39162GSE39162E-GEOD-39162EFO_0000635EFO_0000305E-GEOD-39162