biostudies-otherUnknownLangenfeld JohnHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-49281EurocanPlatformH1299 cells were stably transfected with the Oct4 promoter/GFP or Nestin promoter/GFP reporter vectors. By FACS, 106 cells expressing high levels of GFP were isolated and placed into cell culture for twenty-four hours. Total RNA was used. Bone morphogenetic proteins (BMP) are aberrantly expressed in most lung carcinomas. BMPs mediate cell fate decisions and self-renewal of stem cells. Inhibition of BMP signaling decreases the growth and induces cell death of lung cancer cells lines. It is not known whether the BMP signaling cascade is growth promoting in lung cancer cells expressing the stem cell markers Oct4 and/or nestin. Lung cancer cells expressing Oct4 or nestin were isolated from lung cancer cell lines by stably transfecting the Oct4 promoter or Nestin promoter expression vectors thabiostudies-othertranscription profiling by arrayEurocanPlatformLangenfeld ElaineZachariah EmmanuelLangenfeld JohnDeen MalikfalseBone Morphogenetic Protein Receptor Antagonists Inhibit Growth and Survival of Lung Cancer Cells Expressing Stem Cell Markers Oct4 or NestinH1299 cells were stably transfected with the Oct4 promoter/GFP or Nestin promoter/GFP reporter vectors. By FACS, 106 cells expressing high levels of GFP were isolated and placed into cell culture for twenty-four hours. Total RNA was used. Bone morphogenetic proteins (BMP) are aberrantly expressed in most lung carcinomas. BMPs mediate cell fate decisions and self-renewal of stem cells. Inhibition of BMP signaling decreases the growth and induces cell death of lung cancer cells lines. It is not known whether the BMP signaling cascade is growth promoting in lung cancer cells expressing the stem cell markers Oct4 and/or nestin. Lung cancer cells expressing Oct4 or nestin were isolated from lung cancer cell lines by stably transfecting the Oct4 promoter or Nestin promoter expression vectors tha2016-04-14T14:27:59Z2016-04-14T14:27:59Z2016-04-14T14:27:59ZS-ECPF-GEOD-49281GSE49281E-GEOD-49281EFO_0000322EFO_0001071E-GEOD-49281