<HashMap><database>biostudies-other</database><scores/><additional><omics_type>Unknown</omics_type><submitter>Xu Jia</submitter><species>Homo sapiens</species><full_dataset_link>https://www.ebi.ac.uk/biostudies/studies/S-ECPF-GEOD-52066</full_dataset_link><project>EurocanPlatform</project><abstract>To explore the regulatory role of 14-3-3ζ in TGF-β induced bone metastasis program in 231 cells, we generated MDA-MB-231 human breast cancer cell sublines with 14-3-3ζ shRNA knockdown (231.ZKD-4 and 231.ZKD-5) and scrambled shRNA (231.Scr) We performed cDNA microarray analysis on these cells treated with vehicle or TGF-β (5ng/ml, 2 hours) respectively in vitro Total RNA were extracted from 231.scr, 231.ZKD-4, 231.ZKD-5 cells treated with vehicle or TGF-β, and subjected to illumina Human HT-12 v4 arrays analysis</abstract><repository>biostudies-other</repository><experiment_type>transcription profiling by array</experiment_type><data_source>EurocanPlatform</data_source><pubmed_authors>Xu Jia</pubmed_authors><pubmed_authors>Yu Dihua</pubmed_authors></additional><is_claimable>false</is_claimable><name>14-3-3? promotes TGF-?-induced bone metastasis program in 231 cells</name><description>To explore the regulatory role of 14-3-3ζ in TGF-β induced bone metastasis program in 231 cells, we generated MDA-MB-231 human breast cancer cell sublines with 14-3-3ζ shRNA knockdown (231.ZKD-4 and 231.ZKD-5) and scrambled shRNA (231.Scr) We performed cDNA microarray analysis on these cells treated with vehicle or TGF-β (5ng/ml, 2 hours) respectively in vitro Total RNA were extracted from 231.scr, 231.ZKD-4, 231.ZKD-5 cells treated with vehicle or TGF-β, and subjected to illumina Human HT-12 v4 arrays analysis</description><dates><release>2016-04-14T14:31:45Z</release><modification>2016-04-14T14:31:45Z</modification><creation>2016-04-14T14:31:45Z</creation></dates><accession>S-ECPF-GEOD-52066</accession><cross_references><GEO>GSE52066</GEO><ArrayExpress>E-GEOD-52066</ArrayExpress><EFO>EFO_0000305</EFO><EFO>EFO_0000322</EFO><ArrayExpress files>E-GEOD-52066</ArrayExpress files></cross_references></HashMap>