{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"submitter":["Gbelcová H"],"pagination":["126"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-ECPF-MTAB-1501"],"project":["EurocanPlatform"],"abstract":["The aim of our study was to assess the impact of simvastatin on the amount of cytosolic lipid droplets (LDs), which are implicated in many biological processes including proliferation, inflammation, carcinogenesis, apoptosis, necrosis or growth arrest. Human  pancreatic cancer cells MiaPaCa-2 were treated with simvastatin (6 and 12 _M) for 24 hours. Changes in expression of genes related to lipid metabolism in the simvastatin-treated cells were examined by DNA microarray analysis. The treatment of the cells with simvastatin increased their intracellular content of LDs, partially due to the uptake of cholesterol and triacylglycerides from medium; but in particular, due to enhanced synthesis of triacylglycerides as proved by detection of significant overexpression of genes related to de novo"],"repository":["biostudies-other"],"experiment_type":["transcription profiling by array"],"data_source":["EurocanPlatform"],"omics_type":["Unknown"],"volume":["12"],"journal":["Lipids in health and disease"],"species":["Homo sapiens"],"pubmed_authors":["Strnad H","Svéda M","Kolář M","Ruml T","Böhmer D","Vítek L","Zelenka J","Gbelcová H","Laubertová L","Varga I"],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling by array of human MiaPaCa-2 pancreatic cancer cells to invesitgate effect of simvastatin","description":"The aim of our study was to assess the impact of simvastatin on the amount of cytosolic lipid droplets (LDs), which are implicated in many biological processes including proliferation, inflammation, carcinogenesis, apoptosis, necrosis or growth arrest. Human  pancreatic cancer cells MiaPaCa-2 were treated with simvastatin (6 and 12 _M) for 24 hours. Changes in expression of genes related to lipid metabolism in the simvastatin-treated cells were examined by DNA microarray analysis. The treatment of the cells with simvastatin increased their intracellular content of LDs, partially due to the uptake of cholesterol and triacylglycerides from medium; but in particular, due to enhanced synthesis of triacylglycerides as proved by detection of significant overexpression of genes related to de novo","dates":{"release":"2016-04-14T14:48:55Z","publication":"2013","modification":"2016-04-14T14:48:55Z","creation":"2016-04-14T14:48:55Z"},"accession":"S-ECPF-MTAB-1501","cross_references":{"ArrayExpress":["E-MTAB-1501"],"EFO":["EFO_0000322","EFO_0002618"],"ArrayExpress files":["E-MTAB-1501"]}}