{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"submitter":["Le Floc'h A"],"pagination":["559-570"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-ECPF-TABM-208"],"project":["EurocanPlatform","EurocanPlatform core"],"abstract":["The IGR-Heu tumor cell line was established from patient Heu suffering from large cell carcinoma of the lung. Heu171, Heu127 and Heu161 T cell clones were isolated from autologous tumor infiltrating lymphocytes. H32 T cell line and H32-22 clone were isolated from autologous PBL after stimulation with IGR-Heu and sorting with peptide-MHC tetramers. T cells were grown in the presence of irradiated autologous tumor and lymphoblastoid cell lines in complete media supplemented with rIL-2 and conditioned medium from phytohemagglutinin-activated lymphocytes for 3 weeks. Then, for microarray assays, RNA was extracted from the different cells."],"repository":["biostudies-other"],"experiment_type":["transcription profiling by array"],"data_source":["EurocanPlatform"],"omics_type":["Unknown"],"volume":["204"],"journal":["The Journal of experimental medicine"],"species":["Homo sapiens"],"pubmed_authors":["Mami-Chouaib F","Le Floc'h A","Chouaib S","Lazar V","Bismuth G","Vergnon I","Jalil A","Le Maux Chansac B"],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling of human T cell lines isolated from tumor infiltrating lymphocytes or peripheral blood lymphocytes of a patient with large cell carcinoma of the lung","description":"The IGR-Heu tumor cell line was established from patient Heu suffering from large cell carcinoma of the lung. Heu171, Heu127 and Heu161 T cell clones were isolated from autologous tumor infiltrating lymphocytes. H32 T cell line and H32-22 clone were isolated from autologous PBL after stimulation with IGR-Heu and sorting with peptide-MHC tetramers. T cells were grown in the presence of irradiated autologous tumor and lymphoblastoid cell lines in complete media supplemented with rIL-2 and conditioned medium from phytohemagglutinin-activated lymphocytes for 3 weeks. Then, for microarray assays, RNA was extracted from the different cells.","dates":{"release":"2016-04-14T14:53:48Z","publication":"2007 Mar","modification":"2016-04-14T14:53:48Z","creation":"2016-04-14T14:53:48Z"},"accession":"S-ECPF-TABM-208","cross_references":{"ArrayExpress":["E-TABM-208"],"EFO":["EFO_0003050","EFO_0000322"],"ArrayExpress files":["E-TABM-208"]}}