biostudies-otherLe Floc'h A559-570https://www.ebi.ac.uk/biostudies/studies/S-ECPF-TABM-208EurocanPlatformEurocanPlatform coreThe IGR-Heu tumor cell line was established from patient Heu suffering from large cell carcinoma of the lung. Heu171, Heu127 and Heu161 T cell clones were isolated from autologous tumor infiltrating lymphocytes. H32 T cell line and H32-22 clone were isolated from autologous PBL after stimulation with IGR-Heu and sorting with peptide-MHC tetramers. T cells were grown in the presence of irradiated autologous tumor and lymphoblastoid cell lines in complete media supplemented with rIL-2 and conditioned medium from phytohemagglutinin-activated lymphocytes for 3 weeks. Then, for microarray assays, RNA was extracted from the different cells.biostudies-othertranscription profiling by arrayEurocanPlatformUnknown204The Journal of experimental medicineHomo sapiensMami-Chouaib FLe Floc'h AChouaib SLazar VBismuth GVergnon IJalil ALe Maux Chansac BfalseTranscription profiling of human T cell lines isolated from tumor infiltrating lymphocytes or peripheral blood lymphocytes of a patient with large cell carcinoma of the lungThe IGR-Heu tumor cell line was established from patient Heu suffering from large cell carcinoma of the lung. Heu171, Heu127 and Heu161 T cell clones were isolated from autologous tumor infiltrating lymphocytes. H32 T cell line and H32-22 clone were isolated from autologous PBL after stimulation with IGR-Heu and sorting with peptide-MHC tetramers. T cells were grown in the presence of irradiated autologous tumor and lymphoblastoid cell lines in complete media supplemented with rIL-2 and conditioned medium from phytohemagglutinin-activated lymphocytes for 3 weeks. Then, for microarray assays, RNA was extracted from the different cells.2016-04-14T14:53:48Z2007 Mar2016-04-14T14:53:48Z2016-04-14T14:53:48ZS-ECPF-TABM-208E-TABM-208EFO_0003050EFO_0000322E-TABM-208