{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["275 ( Pt 3)"],"submitter":["Rogers KR"],"journal":["The Biochemical journal"],"pagination":["789-91"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1150123"],"abstract":["Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts."],"repository":["biostudies-other"],"data_source":["Europe PMC"],"pubmed_authors":["Morris CJ","Blake DR","Rogers KR"],"additional_accession":[]},"is_claimable":false,"name":"Oxidation of thiol in the vimentin cytoskeleton.","description":"Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts.","dates":{"release":"1991-01-01T00:00:00Z","publication":"1991 May","modification":"2019-08-04T07:19:48Z","creation":"2019-08-04T07:19:48Z"},"accession":"S-EPMC1150123","cross_references":{"DOI":["10.1042/bj2750789 "]}}