biostudies-otherUnknown275 ( Pt 3)Rogers KRThe Biochemical journal789-91https://www.ebi.ac.uk/biostudies/studies/S-EPMC1150123Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts.biostudies-otherEurope PMCMorris CJBlake DRRogers KRfalseOxidation of thiol in the vimentin cytoskeleton.Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts.1991-01-01T00:00:00Z1991 May2019-08-04T07:19:48Z2019-08-04T07:19:48ZS-EPMC115012310.1042/bj2750789