{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["94"],"submitter":["GLYNN IM"],"journal":["The Biochemical journal"],"pagination":["692-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1206604"],"abstract":["1. Subcellular fractions were prepared from the electric organs of Electrophorus and Torpedo and assayed for adenosine-triphosphatase activity. 2. Treatment of the ;low-speed' fraction from Torpedo with m-urea gave an adenosine-triphosphatase preparation that was almost completely (98%) inhibited by ouabain (0.1mg./ml.) and dependent on the simultaneous presence of Na(+) and K(+). 3. The adenosine-triphosphatase preparations were exposed to [gamma-(32)P]ATP for 30sec. in the presence of (i) Na(+), (ii) K(+), (iii) Na(+)+K(+) and (iv) Na(+)+K(+)+ouabain. No significant labelling of phosphatidic acid, triphosphoinositide or any other phospholipid was observed. 4. The results suggest that phospholipids do not act as phosphorylated intermediates in the ;transport adenosine-triphosphatase' system of electric organ."],"repository":["biostudies-other"],"data_source":["Europe PMC"],"pubmed_authors":["DAWSON RM","EICHBERG J","GLYNN IM","SLAYMAN CW"],"additional_accession":[]},"is_claimable":false,"name":"THE ADENOSINE-TRIPHOSPHATASE SYSTEM RESPONSIBLE FOR CATION TRANSPORT IN ELECTRIC ORGAN: EXCLUSION OF PHOSPHOLIPIDS AS INTERMEDIATES.","description":"1. Subcellular fractions were prepared from the electric organs of Electrophorus and Torpedo and assayed for adenosine-triphosphatase activity. 2. Treatment of the ;low-speed' fraction from Torpedo with m-urea gave an adenosine-triphosphatase preparation that was almost completely (98%) inhibited by ouabain (0.1mg./ml.) and dependent on the simultaneous presence of Na(+) and K(+). 3. The adenosine-triphosphatase preparations were exposed to [gamma-(32)P]ATP for 30sec. in the presence of (i) Na(+), (ii) K(+), (iii) Na(+)+K(+) and (iv) Na(+)+K(+)+ouabain. No significant labelling of phosphatidic acid, triphosphoinositide or any other phospholipid was observed. 4. The results suggest that phospholipids do not act as phosphorylated intermediates in the ;transport adenosine-triphosphatase' system of electric organ.","dates":{"release":"1965-01-01T00:00:00Z","publication":"1965 Mar","modification":"2019-08-04T07:06:25Z","creation":"2019-08-04T07:06:25Z"},"accession":"S-EPMC1206604","cross_references":{"DOI":["10.1042/bj0940692 "]}}