{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["18(1)"],"submitter":["Timmers HT"],"journal":["Nucleic acids research"],"pagination":["23-34"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC330199"],"abstract":["We have cloned the immediate-early serum-reponsive JE gene from the rat in order to study the regulation of this gene. We show that sequences of the JE promoter region confer serum-inducibility to a reporter gene. Analysis of the promoter in transient assays reveals that: i) the -141/-88 region is required for the response to the phorbol ester TPA, ii) the -70/-38 region is essential for basal activity. This latter region harbors the sequence TGACTCC, which resembles the consensus site for AP-1 binding, TGACTCA. DNA-protein binding assays indicate that the JE AP-1 site and the consensus AP-1 site have an overlapping but not identical binding spectrum for AP-1 proteins. Our data suggest that the inability of some AP-1 sites to respond to TPA is caused by subtle differences in affinity for AP-1 proteins."],"repository":["biostudies-other"],"pmcid":["PMC330199"],"data_source":["Europe PMC"],"pubmed_authors":["Pronk GJ","van der Eb AJ","Bos JL","Timmers HT"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of the rat JE gene promoter identifies an AP-1 binding site essential for basal expression but not for TPA induction.","description":"We have cloned the immediate-early serum-reponsive JE gene from the rat in order to study the regulation of this gene. We show that sequences of the JE promoter region confer serum-inducibility to a reporter gene. Analysis of the promoter in transient assays reveals that: i) the -141/-88 region is required for the response to the phorbol ester TPA, ii) the -70/-38 region is essential for basal activity. This latter region harbors the sequence TGACTCC, which resembles the consensus site for AP-1 binding, TGACTCA. DNA-protein binding assays indicate that the JE AP-1 site and the consensus AP-1 site have an overlapping but not identical binding spectrum for AP-1 proteins. Our data suggest that the inability of some AP-1 sites to respond to TPA is caused by subtle differences in affinity for AP-1 proteins.","dates":{"release":"1990-01-01T00:00:00Z","publication":"1990 Jan","modification":"2019-03-27T00:50:02Z","creation":"2019-03-27T00:50:02Z"},"accession":"S-EPMC330199","cross_references":{"gen":["X17053"],"pubmed":["2106664"],"doi":["10.1093/nar/18.1.23 "]}}