biostudies-otherConde CNEI NIH HHSNIDA NIH HHS6793-800https://www.ebi.ac.uk/biostudies/studies/S-EPMC3845415RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation.biostudies-otherEurope PMCUnknown30(19)The Journal of neuroscience : the official journal of the Society for NeurosciencePMC3845415P01 DA010044R01 EY11307R01 EY011307R01 EY011307-04DA10044Caceres ASung CHRobin MChuang JZLi ASaito MNairn ACConde CArias CfalseEvidence for the involvement of Lfc and Tctex-1 in axon formation.RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation.2010-01-01T00:00:00Z2010 May2019-03-27T03:09:32Z2019-03-27T03:09:32ZS-EPMC38454152046324110.1523/JNEUROSCI.5420-09.2010