{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"submitter":["Kim SJ"],"funding":["NCI NIH HHS"],"pagination":["6902-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4159431"],"abstract":["Fully manual use of conventional multiwell plates makes enzyme-linked immunosorbent assay (ELISA)-based immunoassays highly time-consuming and labor-intensive. Here, we present a capillarity-driven on-chip immunoassay that greatly saves time and labor with an inexpensive setup. Our immunoassay process starts with pipetting multiple solutions into multiwells constructed on a microfluidic device chip. Subsequently, capillarity spontaneously transports multiple sample solutions and common reagent solutions into assigned detection channels on the chip in a purely passive and preprogrammed manner. Our device implements capillarity-driven immunoassays involving four sample and six reagent solutions within 30 min by orchestrating the functions of on-chip passive components. Notably, our immunoassay technique reduces the total number of pipetting processes by ~5 times, as compared to assays on multiwell plates (48 vs 10). This assay technique allows us to quantify the concentrations of C-reactive protein and suppressor of tumorigenicity 2 with a detection limit of 8 and 90 pM, respectively. This device should be useful for sophisticated, parallel biochemical microfluidic processing in point-of-care settings under limited resources."],"repository":["biostudies-other"],"data_source":["Europe PMC"],"omics_type":["Unknown"],"volume":["85(14)"],"journal":["Analytical chemistry"],"pmcid":["PMC4159431"],"funding_grant_id":["R01 CA142750","R01-CA-142750-01"],"pubmed_authors":["Takayama S","Paczesny S","Kim SJ","Kurabayashi K"],"additional_accession":[]},"is_claimable":false,"name":"Preprogrammed, parallel on-chip immunoassay using system-level capillarity control.","description":"Fully manual use of conventional multiwell plates makes enzyme-linked immunosorbent assay (ELISA)-based immunoassays highly time-consuming and labor-intensive. Here, we present a capillarity-driven on-chip immunoassay that greatly saves time and labor with an inexpensive setup. Our immunoassay process starts with pipetting multiple solutions into multiwells constructed on a microfluidic device chip. Subsequently, capillarity spontaneously transports multiple sample solutions and common reagent solutions into assigned detection channels on the chip in a purely passive and preprogrammed manner. Our device implements capillarity-driven immunoassays involving four sample and six reagent solutions within 30 min by orchestrating the functions of on-chip passive components. Notably, our immunoassay technique reduces the total number of pipetting processes by ~5 times, as compared to assays on multiwell plates (48 vs 10). This assay technique allows us to quantify the concentrations of C-reactive protein and suppressor of tumorigenicity 2 with a detection limit of 8 and 90 pM, respectively. This device should be useful for sophisticated, parallel biochemical microfluidic processing in point-of-care settings under limited resources.","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Jul","modification":"2019-03-27T01:35:27Z","creation":"2019-03-27T01:35:27Z"},"accession":"S-EPMC4159431","cross_references":{"pubmed":["23789820"],"doi":["10.1021/ac401292d "]}}