{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"submitter":["Warren PM"],"funding":["Wings for Life (Wings for Life Research Foundation)","International Spinal Research Trust (Spinal Research)","U.S. Department of Health & Human Services | National Institutes of Health (NIH)","Craig H. Neilsen Foundation"],"pagination":["4843"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6258702"],"abstract":["There exists an abundance of barriers that hinder functional recovery following spinal cord injury, especially at chronic stages. Here, we examine the rescue of breathing up to 1.5 years following cervical hemisection in the rat. In spite of complete hemidiaphragm paralysis, a single injection of chondroitinase ABC in the phrenic motor pool restored robust and persistent diaphragm function while improving neuromuscular junction anatomy. This treatment strategy was more effective when applied chronically than when assessed acutely after injury. The addition of intermittent hypoxia conditioning further strengthened the ventilatory response. However, in a sub-population of animals, this combination treatment caused excess serotonergic (5HT) axon sprouting leading to aberrant tonic activity in the diaphragm that could be mitigated via 5HT2 receptor blockade. Through unmasking of the continuing neuroplasticity that develops after injury, our treatment strategy ensured rapid and robust patterned respiratory recovery after a near lifetime of paralysis."],"repository":["biostudies-other"],"data_source":["Europe PMC"],"omics_type":["Unknown"],"volume":["9(1)"],"journal":["Nature communications"],"pmcid":["PMC6258702"],"funding_grant_id":["STR117","221988","WFL-US-027/14","R21OD018297","R01NS101105","NS025713"],"pubmed_authors":["Alilain WJ","Steiger SC","Dick TE","Warren PM","Silver J","MacFarlane PM"],"additional_accession":[]},"is_claimable":false,"name":"Rapid and robust restoration of breathing long after spinal cord injury.","description":"There exists an abundance of barriers that hinder functional recovery following spinal cord injury, especially at chronic stages. Here, we examine the rescue of breathing up to 1.5 years following cervical hemisection in the rat. In spite of complete hemidiaphragm paralysis, a single injection of chondroitinase ABC in the phrenic motor pool restored robust and persistent diaphragm function while improving neuromuscular junction anatomy. This treatment strategy was more effective when applied chronically than when assessed acutely after injury. The addition of intermittent hypoxia conditioning further strengthened the ventilatory response. However, in a sub-population of animals, this combination treatment caused excess serotonergic (5HT) axon sprouting leading to aberrant tonic activity in the diaphragm that could be mitigated via 5HT2 receptor blockade. Through unmasking of the continuing neuroplasticity that develops after injury, our treatment strategy ensured rapid and robust patterned respiratory recovery after a near lifetime of paralysis.","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Nov","modification":"2019-03-27T00:10:07Z","creation":"2019-03-27T00:10:07Z"},"accession":"S-EPMC6258702","cross_references":{"pubmed":["30482901"],"doi":["10.1038/s41467-018-06937-0 "]}}