{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Dr. Chris, Andrew Brosnan"],"funding":["European Molecular Biology Organization (EMBO)","EC | H2020 | H2020 Priority Excellent Science | H2020 European Research Council (ERC)"],"journal":["The EMBO Journal"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-SCDT-EMBOJ-2018-100754"],"abstract":["Loaded into ARGONAUTE(AGO) proteins, eukaryotic micro(mi)RNAs regulate gene expression via cleavage, translational repression and/or accelerated decay of sequence-complementary target transcripts. Despite their importance in development, cell identity-maintenance and stress responses, how individual miRNAs contribute to spatial gene regulation within the complex cell mosaics formed in tissues/organs has remained inaccessible in any organism to date. We have developed a non-invasive methodology to examine, at single-cell-type resolution, the AGO-loading and activity patterns of entire miRNA cohorts in intact organs, applied here to the Arabidopsis root-tip. A dual miRNAome-targetome analytical interface allowing intuitive data integration/visualization was developed as the basis for in-dept"],"repository":["biostudies-other"],"funding_grant_id":["ALTF 728-2009","323071"],"pubmed_authors":["Dr. Chris, Andrew Brosnan","Nicolas, Gerardo Bologna","Alexis Sarazin","Prof. Olivier Voinnet","Peiqi Lim","Matthias Hirsch-Hoffmann"],"additional_accession":[]},"is_claimable":false,"name":"Genome-scale, single cell-type resolution of microRNA activities within a whole plant organ","description":"Loaded into ARGONAUTE(AGO) proteins, eukaryotic micro(mi)RNAs regulate gene expression via cleavage, translational repression and/or accelerated decay of sequence-complementary target transcripts. Despite their importance in development, cell identity-maintenance and stress responses, how individual miRNAs contribute to spatial gene regulation within the complex cell mosaics formed in tissues/organs has remained inaccessible in any organism to date. We have developed a non-invasive methodology to examine, at single-cell-type resolution, the AGO-loading and activity patterns of entire miRNA cohorts in intact organs, applied here to the Arabidopsis root-tip. A dual miRNAome-targetome analytical interface allowing intuitive data integration/visualization was developed as the basis for in-dept","dates":{"release":"2020-04-01T00:00:00Z","modification":"2020-04-01T12:31:25Z","creation":"2020-04-01T12:31:25Z"},"accession":"S-SCDT-EMBOJ-2018-100754","cross_references":{"doi":["10.15252/embj.2018100754"]}}