biostudies-otherUnknownDr. Chris, Andrew BrosnanEuropean Molecular Biology Organization (EMBO)EC | H2020 | H2020 Priority Excellent Science | H2020 European Research Council (ERC)The EMBO Journalhttps://www.ebi.ac.uk/biostudies/studies/S-SCDT-EMBOJ-2018-100754Loaded into ARGONAUTE(AGO) proteins, eukaryotic micro(mi)RNAs regulate gene expression via cleavage, translational repression and/or accelerated decay of sequence-complementary target transcripts. Despite their importance in development, cell identity-maintenance and stress responses, how individual miRNAs contribute to spatial gene regulation within the complex cell mosaics formed in tissues/organs has remained inaccessible in any organism to date. We have developed a non-invasive methodology to examine, at single-cell-type resolution, the AGO-loading and activity patterns of entire miRNA cohorts in intact organs, applied here to the Arabidopsis root-tip. A dual miRNAome-targetome analytical interface allowing intuitive data integration/visualization was developed as the basis for in-deptbiostudies-otherALTF 728-2009323071Dr. Chris, Andrew BrosnanNicolas, Gerardo BolognaAlexis SarazinProf. Olivier VoinnetPeiqi LimMatthias Hirsch-HoffmannfalseGenome-scale, single cell-type resolution of microRNA activities within a whole plant organLoaded into ARGONAUTE(AGO) proteins, eukaryotic micro(mi)RNAs regulate gene expression via cleavage, translational repression and/or accelerated decay of sequence-complementary target transcripts. Despite their importance in development, cell identity-maintenance and stress responses, how individual miRNAs contribute to spatial gene regulation within the complex cell mosaics formed in tissues/organs has remained inaccessible in any organism to date. We have developed a non-invasive methodology to examine, at single-cell-type resolution, the AGO-loading and activity patterns of entire miRNA cohorts in intact organs, applied here to the Arabidopsis root-tip. A dual miRNAome-targetome analytical interface allowing intuitive data integration/visualization was developed as the basis for in-dept2020-04-01T00:00:00Z2020-04-01T12:31:25Z2020-04-01T12:31:25ZS-SCDT-EMBOJ-2018-10075410.15252/embj.2018100754