biostudies-otherUnknownProf. Nancy PapalopuluWellcome Trust (WT)The EMBO Journalhttps://www.ebi.ac.uk/biostudies/studies/S-SCDT-EMBOJ-2019-103558PNoise is prevalent in biology and has been widely quantified using snapshot measurements. This static view obscures our understanding of dynamic noise properties and how these affect gene expression and cell state transitions. Using a CRISPR/Cas9 Zebrafish Her6::Venus reporter combined with mathematical and in vivo experimentation, we explore how noise affects the protein dynamics of Her6, a basic helix-loop-helix transcriptional repressor. During neurogenesis, Her6 expression transitions from fluctuating to oscillatory at single cell level. We identify that absence of miR-9 input generates high frequency noise in Her6 traces, inhibits the transition to oscillatory protein expression and prevents the downregulation of Her6. Together, these impair the upregulation of downstream targets and biostudies-otherR118623Ms. Parnian DoostdarDr. Veronica BigaMr. Robert LeaMs. Riba ThomasDr. Jochen KursaweProf. Nancy PapalopuluXimena SotofalseDynamic properties of noise and Her6 levels are optimized by miR-9, allowing the decoding of the Her6 oscillatorNoise is prevalent in biology and has been widely quantified using snapshot measurements. This static view obscures our understanding of dynamic noise properties and how these affect gene expression and cell state transitions. Using a CRISPR/Cas9 Zebrafish Her6::Venus reporter combined with mathematical and in vivo experimentation, we explore how noise affects the protein dynamics of Her6, a basic helix-loop-helix transcriptional repressor. During neurogenesis, Her6 expression transitions from fluctuating to oscillatory at single cell level. We identify that absence of miR-9 input generates high frequency noise in Her6 traces, inhibits the transition to oscillatory protein expression and prevents the downregulation of Her6. Together, these impair the upregulation of downstream targets and 2021-08-06T00:00:00Z2021-08-06T20:00:35Z2021-08-06T20:00:35ZS-SCDT-EMBOJ-2019-103558P10.15252/embj.2019103558