biostudies-otherUnknownProf. Artur ScherfParaFrap LabExFOSISS-CONACyTConsejo Nacional de Ciencia y Tecnología (CONACYT)European Research Council Advanced grantEMBO Reportshttps://www.ebi.ac.uk/biostudies/studies/S-SCDT-EMBOR-2018-46331-TPost-translational modifications of histone H3 N-terminal tails are key epigenetic regulators of virulence gene expression and sexual commitment in the human malaria parasite Plasmodium falciparum. Here, we identify proteolytic clipping of the N-terminal tail of nucleosome-associated histone H3 at amino acid position 21 as a new chromatin modification. A Cathepsin C-like proteolytic clipping activity is observed in nuclear parasite extracts. Notably, an ectopically expressed version of clipped histone H3, PfH3p-HA, is targeted to the nucleus and integrates into mononucleosomes. Furthermore, chromatin immunoprecipitation and next generation sequencing analysis identified PfH3p-HA as being highly enriched in the upstream region of six genes that play a key role in DNA replication and repair:biostudies-other228896272364ANR-11-LABX0024PlasmoSilencing 670301Dr. Beatriz Xoconostle-CazaresDr. Gabriela, Romero MezaDr. Shruthi, Shidhar VembarDr. Rafael, Miyazawa MartinsRosaura Hernandez-RivasProf. Artur ScherfMs. Daniela Lozano-AmadoMiguel VargasDr. Cameron, Ross MacPhersonDr. Abril, Marcela Herrera-SolorioPatty ChenfalseClipped histone H3 is integrated into nucleosomes of DNA replication genes in the human malaria parasite Plasmodium falciparumPost-translational modifications of histone H3 N-terminal tails are key epigenetic regulators of virulence gene expression and sexual commitment in the human malaria parasite Plasmodium falciparum. Here, we identify proteolytic clipping of the N-terminal tail of nucleosome-associated histone H3 at amino acid position 21 as a new chromatin modification. A Cathepsin C-like proteolytic clipping activity is observed in nuclear parasite extracts. Notably, an ectopically expressed version of clipped histone H3, PfH3p-HA, is targeted to the nucleus and integrates into mononucleosomes. Furthermore, chromatin immunoprecipitation and next generation sequencing analysis identified PfH3p-HA as being highly enriched in the upstream region of six genes that play a key role in DNA replication and repair:2020-01-16T21:00:10Z2020-01-16T21:00:10Z2020-01-16T21:00:10ZS-SCDT-EMBOR-2018-46331-T10.15252/embr.201846331