biostudies-otherUnknownDr. Michele BrischigliaroAFM-TéléthonFondazione Telethon (FT)Associazione Luigi Comini ONLUSZonMw (Netherlands Organisation for Health Research and Development)Institut de France (Institute of France)EMBO Reportshttps://www.ebi.ac.uk/biostudies/studies/S-SCDT-EMBOR-2022-54825-TThe mitochondrial respiratory chain (MRC) is composed of four multiheteromeric enzyme complexes. According to the endosymbiotic origin of mitochondria, eukaryotic MRC derives from ancestral proteobacterial respiratory structures consisting of a minimal set of complexes formed by a few subunits associated with redox prosthetic groups. These enzymes, which are the "core" redox centers of respiration, acquired additional subunits and increased their complexity throughout evolution. Cytochrome c oxidase (COX), the terminal component of MRC, has a highly interspecific heterogeneous composition. Mammalian COX consists of 14 different polypeptides, of which COX7B is considered the evolutionarily youngest subunit. We applied proteomic, biochemical, and genetic approaches to investigate the COX combiostudies-otherGGP1900723706NRJ Fondation91217009GGP20013Dr. Erika Fernandez-VizarraProf. Stefano MoroProf. Martijn, A. HuynenDr. Alfredo Cabrera-OreficeMr. Dei, Martinez ElurbeDr. Susanne ArnoldMs. Elena FrigoDr. Mattia SturleseProf. Massimo ZevianiDr. Michele BrischigliaroProf. Carlo ViscomifalseCG7630 is the D. melanogaster homolog of the cytochrome c oxidase subunit COX7BThe mitochondrial respiratory chain (MRC) is composed of four multiheteromeric enzyme complexes. According to the endosymbiotic origin of mitochondria, eukaryotic MRC derives from ancestral proteobacterial respiratory structures consisting of a minimal set of complexes formed by a few subunits associated with redox prosthetic groups. These enzymes, which are the "core" redox centers of respiration, acquired additional subunits and increased their complexity throughout evolution. Cytochrome c oxidase (COX), the terminal component of MRC, has a highly interspecific heterogeneous composition. Mammalian COX consists of 14 different polypeptides, of which COX7B is considered the evolutionarily youngest subunit. We applied proteomic, biochemical, and genetic approaches to investigate the COX com2022-07-10T00:00:00Z2022-07-10T10:37:10.851Z2022-07-10T10:37:10.851ZS-SCDT-EMBOR-2022-54825-T10.15252/embr.202254825