biostudies-otherUnknownDutch Research Agenda (NWA)Homo sapienshttps://www.ebi.ac.uk/biostudies/studies/S-VHPS25biostudies-otherfalseTacrolimus-induced nephrotoxicity in tubuloidsTacrolimus (TAC) is a first-line immunosuppressant used to prevent rejection after solid-organ transplantation, yet its clinical utility is limited by nephrotoxicity, which can progress to irreversible kidney injury or graft loss. The molecular mechanisms underlying TAC-induced nephrotoxicity remain insufficiently understood. Here, we systematically assessed TAC responses in a human in vitro nephron model: adult stem cell-derived kidney tubuloids from two donors (T19 and T30). Cell viability (CellTiter-Fluor), intracellular tacrolimus accumulation (LC-MS/MS), targeted gene expression of drug transporters and metabolizing enzymes (RT-qPCR) and whole-transcriptome responses (bulk RNA-seq) were measured, with co-treatment by P-glycoprotein (P-gp) inhibitors used to modulate intracellular tacrolimus levels.2026-03-18T00:00:00Z2026-06-16T18:30:30.064Z2026-03-18T11:02:12.422ZS-VHPS25