EGAGenomicsMultiomicsAarno Palotiehttps://ega-archive.org/dacs/EGAC00001000096EGAEGA DAC EGAC00001000096EGAap8@sanger.ac.ukDNA methylation is an important mechanism by which gene transcription and hence cellular function are regulated. Here, we provide detailed functional genome-wide methylome maps of 5 primary peripheral blood leukocyte subsets including T cells, B cells, monocytes/macrophages, and neutrophils obtained from healthy individuals. A comparison of these methylomes revealed highly specific cell-lineage and cell-subset methylation profiles. DNA hypomethylation is known to be permissive for gene expression and we identified cell-subset-specific hypomethylated regions (HMRs) that strongly correlate with gene transcription levels suggesting these HMRs may regulate corresponding cell functions. Single-nucleotide polymorphisms associated with immune-mediated disease in genome-wide association studies preferentially localized to these cell-specific regulatory HMRs, offering insight into pathogenesis by highlighting cell subsets in which specific epigenetic changes may drive disease. Our data provide a valuable reference tool for researchers aiming to investigate the role of DNA methylation in regulating primary leukocyte function in health and immune-mediated disease.Genome-wide methylation analyses of primary human leukocyte subsets identifies functionally important cell-type-specific hypomethylated regions.Zilbauer Matthias M, Rayner Tim F TF, Clark Christine C, Coffey Alison J AJ, Joyce Chris J CJ, Palta Priit P, Palotie Aarno A, Lyons Paul A PA, Smith Kenneth G C KGfbwd4, Pilot, l(2)04454, DmelCG1772, dTAF[[II]]230, TAF[[II]]250, d230, dac, CIB1, determination, TAF200, l(3)84Ab, dTAFII250, p21[dacapo], BG:DS00004.13, TAFII-250, TAF250/230, dactylin, EfW1, Cell, dTAF230, E130112M23Rik, cdi4, dmTAF[[II]]230, TAFII250, Pilot Study., dmTAF1, dactylyn, Taf230, p230, p21, chemical analysis, Studies, CG1772, TAF[[II]]250/230, Project, FBWD4, TFIID, Cdi4, CDI4, p27, methylation, Decapo, TAF250, Taf[[II]]250, study, Taf200, dTAF[[II]]250, TAF[[II]]230, TFIID TAF250, Projects, cel, cell, shsf3, shfm3, E(Sev-CycE)2B, Taf1p, TAF[II]250, whole genome, Fbw4, FBW4, CG17603, TAF[[II]], Study, Dach, CDKN2B, dTAF250, Pilot Project, SHFM3, DmelCG17603, Dac, DAC, p27[Dap], Taf250, dacapo/cyclin-dependent kinase interactor 4, SR3-5, AI182278, Dap, CES5A1, P15, assay, fbw4, SHSF3, TAF, Methylations, TAF230, Pilot Studies, TAF1MGC130048, Leukocyte, dTAF[[II]]230, TAF[[II]]250, d230, human being, wide/broad, immune cell, SGCG_HUMAN, 35 kDa dystrophin-associated glycoprotein, Modern, SG-gamma, TAF200, A4, l(3)84Ab, dTAFII250, BG:DS00004.13, broad, PMN cell, TAFII-250, TAF250/230, EfW1, White Blood Corpuscle, Cell, TYPE, SGCG, Human, dTAF230, DAGA4, dmTAF[[II]]230, TAFII250, Homo sapiens, dmTAF1, Taf230, p230, 35DAG, Corpuscles, TAF[[II]]250/230, TFIID, sarcoglycan, LGMD2C., methylation, MAM, gamma-SG, Blood Corpuscle, SCG3, Man, TAF250, Blood Cells, Taf[[II]]250, gamma sarcoglycan, Blood Cell, Taf200, leucocyte, dTAF[[II]]250, TAF[[II]]230, Man (Taxonomy), TFIID TAF250, DMDA1, Genomes, cel, cell, Taf1p, TAF[II]250, whole genome, PMNC, White Blood Corpuscles, CG17603, gamma (35kDa dystrophin-associated glycoprotein), TAF[[II]], human, White Blood Cells, dTAF250, white blood cell, Corpuscle, wide, DmelCG17603, DMDA, Taf250, SR3-5, 35kD dystrophin-associated glycoprotein, Modern Man, White Blood, SCARMD2, gamma-sarcoglycan, White, Blood Corpuscles, TAF, Methylations, White Blood Cell, TAF230, polymorphonuclear cell, TAF1Genome-Wide Association, other disease, d230, Band Cell, Materials, localised, Monocyte Derived Macrophages, Blood, Genome Wide Association Analysis, Whole Genome Association Study, regulation by symbiont of host system process, positive regulation by symbiont of host non-apoptotic programmed cell death, Gene, dTAFII250, DNA Methylations, broad, EfW1, Normalcies, White Blood Corpuscle, Polymorphonuclear, dmTAF[[II]]230, GWA Studies, diseases, dmTAF1, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, Roles, Taf230, PBMC, Monocyte-Derived Macrophage, Studies, disease or disorder, pathogenesis, Concepts, LE Cell, diseases and disorders, Blood Corpuscle, TAF250, Segmented/Neutrophils, Neutrophil Band Cells, Normalities, average, GWA Study, Taf200, human disease, DNA methylation maintenance, dTAF[[II]]250, Genetic, Gene Expressions, localized, TFIID TAF250, stimulation by symbiont of host programmed cell death, Genomes, cel, cell, GWA, Taf1p, Bone Marrow-Derived Macrophage, PMNC, causes, DNA methylation, Expressions, Epigenomic, Genome Wide Association Scan, Genome-Wide Association Studies, non-neoplastic, Bone Marrow-Derived, Study, dTAF250, Monocyte, Neutrophils, Role Concepts, causality, disorder, Homo sapiens disease, Expression, TAF, Nucleotide, transcription from bacterial-type RNA polymerase promoter, LE, Methylations, Neutrophil, Individual Health, Leukocyte, dTAF[[II]]230, TAF[[II]]250, activation by organism of non-apoptotic programmed cell death in other organism, wide/broad, immune cell, whole blood, Polymorphonuclear Neutrophils, hemolysin activity, Leukocytes, disorders, TAF200, Maps, l(3)84Ab, function, Monocyte Derived, BG:DS00004.13, medical condition, PMN cell, Normalcy, TAFII-250, TAF250/230, Cistrons, Genome-Wide, Cell, Polymorphonuclear Leukocyte, dTAF230, Concept, Neutrophil Band Cell, Monocyte-Derived, TAFII250, Role Concept, LE Cells, Genome Wide Association Study, p230, Diseases, modulation by symbiont of host system process, Role, Corpuscles, TAF[[II]]250/230, Genetic Materials, TFIID, condition, methylation, Whole Genome Association Analysis, Individual, Genetic Material, nucleotides, Monocyte-Derived Macrophages, Macrophage, Blood Cells, Taf[[II]]250, Blood Cell, Polymorphonuclear Neutrophil, leucocyte, TAF[[II]]230, Peripheral Blood, Drives, Epigenetic, Normality, Macrophages, Bone Marrow Derived Macrophages, INSDC_feature:gene, TAF[II]250, whole genome, White Blood Corpuscles, CG17603, TAF[[II]], NEUTSGNE, Genome Wide Association Studies, White Blood Cells, Reticuloendothelial System, white blood cell, Corpuscle, disease, wide, Health, DmelCG17603, Taf250, Material, SR3-5, bacterial transcription, PBMCs, activation by symbiont of host programmed cell death, White Blood, Bone Marrow-Derived Macrophages, Polymorphonuclear Leukocytes, Association Studies, Cistron, White, medical condition., Blood Corpuscles, Association Study, DNA, Epigenetics, White Blood Cell, Methylation, TAF230, polymorphonuclear cell, TAF1falseDAC for study Sequencing component for the whole genome methylation analysis in PBMCs and cell subsets pilot study 1733-sc-2013-Data Access Committee EGAC00001000096EGAC00001000096960624159175EGAS00001000490EGAD00001000394