{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"contact_person":["Christian Conrad"],"full_dataset_link":["https://ega-archive.org/dacs/EGAC00001000594"],"host":["EGA"],"description":["EGA DAC EGAC00001000594"],"repository":["EGA"],"email":["christian.conrad@bihealth.de"],"pubmed_abstract":["The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While TMPRSS2 is strongly expressed in both tissues, in the subsegmental bronchial branches ACE2 is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS-CoV-2 infection. Our data provide a rich resource for future investigations of COVID-19 infection and pathogenesis."],"pubmed_title":["SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells."],"pubmed_authors":["Lukassen Soeren S, Chua Robert Lorenz RL, Trefzer Timo T, Kahn Nicolas C NC, Schneider Marc A MA, Muley Thomas T, Winter Hauke H, Meister Michael M, Veith Carmen C, Boots Agnes W AW, Hennig Bianca P BP, Kreuter Michael M, Conrad Christian C, Eils Roland R"],"name_synonyms":["fbwd4, l(2)04454, DmelCG1772, dac, CIB1, shsf3, shfm3, Cell Lines, E(Sev-CycE)2B, patient, p21[dacapo], Fbw4, FBW4, dactylin, Client, Cell, E130112M23Rik, Dach, CDKN2B, cdi4, SHFM3, Dac, DAC, p27[Dap], Patient, dactylyn, dacapo/cyclin-dependent kinase interactor 4, Clients, AI182278, p21, Line, CG1772, FBWD4, Dap, Cdi4, CDI4, CES5A1, Cell., p27, P15, fbw4, SHSF3, Decapo, Lines"],"pubmed_title_synonyms":["COVID19, SARS-CoV-2 Viruses, 2019 Novel Coronavirus, β-CoV, beta-CoV, COVID-19 Viruses, COVID-19, SARS-CoV-2, 2019 novel coronavirus, Coronavirus 2, SARS CoV 2 Virus, Migrant Workers, COVID19 Virus, COVID19 Viruses, Severe Acute Respiratory Syndrome Coronavirus 2, SARS, SARS-CoV-2 Virus, Migrant, Novel Coronavirus, Virus, Nomad, Coronavirus, Cell., Workers, β-coronavirus, 2019-nCoV, Migrants and Transients, 2019 Novel Coronaviruses, SARS Coronavirus 2, β-CoVs, 2019 Novel, betacoronavirus, Migrant Worker, Viruses, Coronavirus Disease 2019 Virus, Transients, Wuhan Seafood Market Pneumonia Virus, Nonmigrant, Nonmigrants, severe acute respiratory syndrome coronavirus 2, Wuhan, Worker, beta-CoVs, 2010305L05Rik, ACEH, Transient, SARS-coronavirus 2, Squatter, COVID-19 Virus, Nomads, UNQ868/PRO1885, Squatters, 2019, Migrants, COVID 19 Virus, Wuhan Coronavirus"],"pubmed_abstract_synonyms":["host organism, dlmo, RNA Sequence Determination, DLMO, supply, Sequence Determination, Rho A, RNA Sequence, positive regulation by symbiont of host non-apoptotic programmed cell death, Infestations and Infections, Virus Infection, 2019 novel coronavirus, Virus Disease, 2019 nCoV Disease, hdp-a, dmTAF[[II]]230, Readability, dLmo, dLMO, DmelCG1004, rho-1, parenchyma of lung, 2019-nCoV Disease, Novel Coronavirus, Virus, pathogenesis, Virus Infections, DRho, Analysis, AAF01186, COVID 19 Pandemic, 8416, Dmrho, increased, Man (Taxonomy), Viral Diseases, Migrant Worker, stimulation by symbiont of host programmed cell death, TFIID TAF250, Analyses, cel, Determination, Coronavirus Disease 2019, Tissue, Nonmigrant, DMRHO, Coronavirus Disease-19, beta-CoVs, ACEH, Sequence Determinations, Transient, Community Health, rhoA, RHO1, Rho1, Respiratory Systems, DmelCG8416, Bd, Respiratory, Dlmo, rho1, Squatters, Tissue Donor, fliH, Infections and Infestations, COVID 19 Virus Disease, tracheal system, 2019-nCoV Infections, dTAF[[II]]230, SARS-CoV-2 Viruses, Transplant Donor, β-CoV, Viral, anatomical protrusion, pulmo, DmRHO-A, Rho-1, COVID-19 Viruses, Respiratory system, Modern, Transplant, RHOb, SARS-CoV-2, TAF200, lung parenchyma, Semen Donors, HSC0013, CG1004, Coronavirus 2, 2019 Novel Coronavirus Disease, infections, 2019 nCoV Infection, TAFII-250, COVID 19 Virus Infection, TAF250/230, Dm Rho1, Determinations, rhom, COVID19 Viruses, TAFII250, RhoN19, SARS-CoV-2 Virus, Migrant, RhoA, Diseases, ve, modulation by symbiont of host system process, RHOM, RhoM, Coronavirus, simple tissue, Donors, Workers, COVID-19 Virus Infections, RHOHP1, neoplasms, Migrants and Transients, Ptd, Pandemic, D-Rho1, AI326383, Viral Infections, Coronavirus Disease 2019 Virus, System, Viral Disease, Ve, CG8416, Infection and Infestation, RHO, Rho, CG17603, TAF[[II]], human, DMRHOa, Semen Donor, DMRHOb, respiratory system, Respiratory Tracts, Health, Semen, Taf250, pulmonary, rhomboid/veinlet, spine, SR3-5, activation by symbiont of host programmed cell death, Lmo, LMO, 2019, Preventive Medicine & Public Health, 2019 Novel Coronavirus Infection, Migrants, ARHD, 2019-nCoV Diseases, hdp, Preventive Medicine and Public Health, rho, Ovum, Ovum Donors, Organ Donors, TAF230, accessory, systema respiratorium, Community, COVID19, Dmelrho, d230, human being, DRORHO, regulation by symbiont of host system process, COVID-19, Arhd, COVID-19 Virus Diseases, dTAFII250, SARS CoV 2 Virus, EfW1, supernumerary, Migrant Workers, Rho GTPase, COVID19 Virus, Human, protrusion, SARS, Homo sapiens, DRhoA, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, dmTAF1, Taf230, dttg, SARS CoV 2 Infection, lung, Severe Acute Respiratory Syndrome Coronavirus 2 Infection, causality., CG6500, β-coronavirus, neoplasm, 2019-nCoV, Man, 2019 Novel Coronaviruses, TAF250, Taf200, dRhoA, SARS Coronavirus 2, β-CoVs, dTAF[[II]]250, Viruses, beadex/dLMO, Infections, distribution, cell, ligand, Transients, Wuhan Seafood Market Pneumonia Virus, COVID-19 Virus Infection, Nonmigrants, severe acute respiratory syndrome coronavirus 2, Taf1p, causes, Worker, DRho1, SARS-CoV-2 Infection, 2010305L05Rik, clinical infection, Sequencing, Transplant Donors, DrhoA, OPN2, Squatter, dTAF250, COVID-19 Virus, Infestation and Infection, RNA Sequence Analyses, RhoHP1, UNQ868/PRO1885, causality, Drho1, RNA Sequencing, dRho1, Respiratory Tract, Ovum Donor, TAF, SARS Coronavirus 2 Infection, Wuhan Coronavirus, Viral Infection, Organ, Sequence Analyses, DmelCG6500, Disease, RNA, Rho kinase, TAF[[II]]250, Coronavirus Disease 19, 2019 Novel Coronavirus, activation by organism of non-apoptotic programmed cell death in other organism, beta-CoV, hemolysin activity, RNA Sequence Determinations, l(2)52Fa, Atmungssystem, iks, l(3)84Ab, BG:DS00004.13, function, SARS-CoV-2 Infections, apparatus respiratorius, l(2)k02107b, Cell, dTAF230, COVID-19 Virus Disease, 2019-nCoV Infection, Severe Acute Respiratory Syndrome Coronavirus 2, Public, p230, Infection, Nomad, TAF[[II]]250/230, TFIID, Donor, Apparatus respiratorius, supply and distribution, respiratory tract, Environment, dnRho, Taf[[II]]250, 2019 Novel, betacoronavirus, Disease 2019, TAF[[II]]230, Organ Donor, increased number, E3.10/J3.8, TAF[II]250, Wuhan, Understanding, COVID-19 Pandemics, COVID-19 Pandemic, SARS-coronavirus 2, Tract, hld, present in greater numbers in organism, DmelCG17603, Modern Man, RNA Sequence Analysis, Nomads, COVID 19 Virus, Lungs, n(2)k07236, COVID 19, TAF1"],"additional_accession":[]},"is_claimable":false,"name":"DAC for patient-derived cell line samples","description":"Data Access Committee EGAC00001000594","dates":{"output":"2025-1-9"},"accession":"EGAC00001000594","cross_references":{"TAXONOMY":["9606"],"pubmed":["32246845"],"EGA":["EGAS00001004419","EGAS00001004064","EGAS00001002239","EGAS00001003852","EGAS00001002999","EGAS00001004661","EGAD00001003146","EGAD00001005315","EGAD00001005524","EGAD00001006185","EGAD00001006448","EGAD00001004131"]}}