{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"contact_person":["Riitta Lahesmaa"],"full_dataset_link":["https://ega-archive.org/dacs/EGAC00001001443"],"host":["EGA"],"description":["EGA DAC EGAC00001001443"],"repository":["EGA"],"email":["riitta.lahesmaa@utu.fi"],"pubmed_abstract":["The appearance of type 1 diabetes (T1D)-associated autoantibodies is the first and only measurable parameter to predict progression toward T1D in genetically susceptible individuals. However, autoantibodies indicate an active autoimmune reaction, wherein the immune tolerance is already broken. Therefore, there is a clear and urgent need for new biomarkers that predict the onset of the autoimmune reaction preceding autoantibody positivity or reflect progressive β-cell destruction. Here we report the mRNA sequencing-based analysis of 306 samples including fractionated samples of CD4<sup>+</sup> and CD8<sup>+</sup> T cells as well as CD4<sup>-</sup>CD8<sup>-</sup> cell fractions and unfractionated peripheral blood mononuclear cell samples longitudinally collected from seven children who developed β-cell autoimmunity (case subjects) at a young age and matched control subjects. We identified transcripts, including interleukin 32 (<i>IL32</i>), that were upregulated before T1D-associated autoantibodies appeared. Single-cell RNA sequencing studies revealed that high <i>IL32</i> in case samples was contributed mainly by activated T cells and NK cells. Further, we showed that <i>IL32</i> expression can be induced by a virus and cytokines in pancreatic islets and β-cells, respectively. The results provide a basis for early detection of aberrations in the immune system function before T1D and suggest a potential role for IL32 in the pathogenesis of T1D."],"pubmed_title":["Early Detection of Peripheral Blood Cell Signature in Children Developing β-Cell Autoimmunity at a Young Age."],"pubmed_authors":["Kallionpää Henna H, Somani Juhi J, Tuomela Soile S, Ullah Ubaid U, de Albuquerque Rafael R, Lönnberg Tapio T, Komsi Elina E, Siljander Heli H, Honkanen Jarno J, Härkönen Taina T, Peet Aleksandr A, Tillmann Vallo V, Chandra Vikash V, Anagandula Mahesh Kumar MK, Frisk Gun G, Otonkoski Timo T, Rasool Omid O, Lund Riikka R, Lähdesmäki Harri H, Knip Mikael M, Lahesmaa Riitta R"],"pubmed_title_synonyms":["Autoimmunity, Autoimmune Responses, Autoimmune disorder, dTAF[[II]]230, TAF[[II]]250, d230, RnBP, PBC, Autoimmunities, TAF200, GlcNAc 2-epimerase, l(3)84Ab, dTAFII250, BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Cell, Fs(3)Sz11, dTAF230, dmTAF[[II]]230, TAFII250, N-acetyl-D-glucosamine 2-epimerase, dmTAF1, Taf230, p230, RENBP, TAF[[II]]250/230, AGE., TFIID, autoimmunity, DmF2, Fs(3)Hor, Autoimmune condition, TAF250, lod, Taf[[II]]250, Autoimmune Response, Taf200, DmelCG2684, dTAF[[II]]250, TAF[[II]]230, TFIID TAF250, Autoimmune disease, cel, cell, Taf1p, TAF[II]250, NTef2, Lds, CG17603, Children, TAF[[II]], early, dTAF250, DmelCG17603, Taf250, Horka, SR3-5, PBC cell, CG2684, Fs(3)Horka, renin-binding protein, TAF, TAF230, TAF1"],"name_synonyms":["fbwd4, l(2)04454, DmelCG1772, dac, CIB1, shsf3, shfm3, Decapo., E(Sev-CycE)2B, p21[dacapo], Fbw4, FBW4, dactylin, E130112M23Rik, Dach, CDKN2B, cdi4, SHFM3, Dac, DAC, p27[Dap], dactylyn, dacapo/cyclin-dependent kinase interactor 4, AI182278, p21, CG1772, FBWD4, Dap, Cdi4, CDI4, CES5A1, p27, P15, fbw4, SHSF3"],"pubmed_abstract_synonyms":["Diabetes Type 1, Biological Markers, Viral Marker, mononuclear leukocyte, Surrogate Endpoints, T-cell surface antigen T4/Leu-3, Immune Systems, RNA Sequence Determination, determination, Peripheral Blood Mononuclear Cells, Laboratory, Insulin Dependent Diabetes Mellitus 1, FON1, Sequence Determination, RNA Sequence, Autoimmunities, Biochemical, positive regulation by symbiont of host non-apoptotic programmed cell death, Endpoint, Progress Reports, Serum, FLORAL ORGAN NUMBER 1, Insulin Dependent Diabetes, prevention, dmTAF[[II]]230, Insulin dependent diabetes mellitus, Laboratory Markers, L3T4, Roles, Biological, Summary Report, SUP, Ly-4, Virus, Juvenile onset diabetes mellitus, 1, Immunosuppression (Physiology), Concepts, pathogenesis, not stated as uncontrolled, Peripheral Blood Mononuclear Cell, autoimmunity, insulin dependent diabetes, Analysis, with unspecified complication, Sudden Onset, Fs(3)Hor, Autoimmune, prevention and control, Summary Reports, Non Polyadenylated, Autoimmune condition, Juvenile Onset Diabetes, Autoimmune Response, DmelCG2684, TFIID TAF250, Autoimmune disease, reference sample, stimulation by symbiont of host programmed cell death, Progress Report, Analyses, cel, Determination, Polyadenylated Messenger, Bdr, Sudden-Onset Diabetes, Age of onset, Super protein, NTef2, Sequence Determinations, sp, SNAP-25, preventive measures, Progress, reaction, Mononuclear, Immune, Markers, Field Reports, Autoantibody, Mononuclear Leukocytes, Immunosuppressions (Physiology), Viral Markers, Role Concepts, associated, juvenile diabetes, Type 1 Diabetes Mellitus, dTAF[[II]]230, Juvenile Onset, preventive therapy, Viral, Surrogate Endpoint, T-cell surface antigen T4|Leu-3, Polyadenylated, TAF200, Brittle, uncontrolled, Biochemical Markers, TAFII-250, TAF250/230, PBMC Peripheral Blood Mononuclear Cells, Biologic Marker, results, Determinations, Fs(3)Sz11, Juvenile-Onset, Diabetes mellitus type I, TAFII250, Role Concept, Investigative Report, Marker, RENBP, Insulin-dependent diabetes, Role, modulation by symbiont of host system process, GENA70, immune mediated diabetes, type I diabetes mellitus, Polyadenylated Messenger RNA, Sudden-Onset, Autoimmune Diabetes, IDDM, Non Polyadenylated mRNA, Interleukin, protein_coding_transcript, PBM cell, End Points, IDDM - Insulin-dependent diabetes mellitus, Diabetes mellitus type 1, T-cell surface glycoprotein CD4, p32, System, Field, Sudden-Onset Diabetes Mellitus, Diabetes mellitus type I [insulin dependent type] [IDDM] [juvenile type], CD4mut, Immunologic, Juvenile Diabetes, Laboratory Marker, CG17603, TAF[[II]], Children, Non-Polyadenylated mRNA, early, Type 1, AGE, Report, Tolerance, hyaline, Taf250, Biochemical Marker, SR3-5, Horka, activation by symbiont of host programmed cell death, CG2684, Fs(3)Horka, Insulin-Dependent Diabetes Mellitus 1, Animal Viruses, Poly(A) RNA, Juvenile-Onset Diabetes Mellitus, TAF230, DMI UNSPF NT ST UNCNTRLD, peripheral blood mononuclear cell, FLORAL DEFECTIVE 10, Autoimmune Responses, Ketosis-Prone Diabetes Mellitus, d230, Juvenile diabetes, Type I Diabetes, Clinical Markers, Clinical Marker, regulation by symbiont of host system process, dTAFII250, Diabetes mellitus type 1 (disorder), SUPERMAN, DIABETES MELLITUS TYPE 01, EfW1, Surrogate End Points, Autoimmune Diabete, Surrogate Markers, Investigative, dmTAF1, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, Taf230, Messenger, Insulin-Dependent, PBMC, susceptible, causality., DmF2, Animal, Type I, MAL, TAF250, lod, Biomarker, Taf200, Animal Virus, Insulin-Dependent Diabetes Mellitus, dTAF[[II]]250, Clinical, Viruses, cell, Biological Marker, Type I diabetes, messenger RNA, Taf1p, causes, Sequencing, dTAF250, Brittle Diabetes Mellitus, Mif1, template RNA, Immunologic Markers, RNA Sequence Analyses, Mononuclear Leukocyte, Diabetes Mellitus, Type 1 Diabetes, RNA Sequencing, TAF, Investigative Reports, preceding, Immunologic Marker, Diabetes, Biologic, Controlled, Sequence Analyses, T1DM, Autoimmunity, Autoimmune disorder, RNA, Controlling, TAF[[II]]250, Leukocyte, T1D, activation by organism of non-apoptotic programmed cell death in other organism, Diabete, hemolysin activity, PBMC cell, RnBP, RNA Sequence Determinations, Serum Markers, Leu2, End Point, Messenger RNA, GlcNAc 2-epimerase, l(3)84Ab, BG:DS00004.13, Synaptosomal-associated 25 kDa protein, function, DMI UNSPF UNCNTRLD, Ketosis Prone, type I diabetes, Cell, Immune Marker, dTAF230, Concept, insulin-dependent diabetes mellitus, N-acetyl-D-glucosamine 2-epimerase, Cytokine, T-cell differentiation antigen L3T4, Surrogate End Point, p230, Poly(A)+ mRNA, Research Reports, chemical analysis, Systems, TAF[[II]]250/230, INSDC_feature:mRNA, TFIID, Polyadenylated RNA, Zoophaginae, Mellitus, Biologic Markers, Taf[[II]]250, Peripheral Blood Human Mononuclear Cells, diabetes mellitis type I, Serum Marker, Poly(A)+ RNA, TAF[[II]]230, mRNA, Poly(A) Tail, Surrogate, prophylaxis, Endpoints, Non-Polyadenylated, TAF[II]250, FLO10, Age symptoms begin, HERP, Lds, diabetes mellitis type 1, Surrogate Marker, Ketosis-Prone, type 1 diabetes, DmelCG17603, clear, Juvenile-Onset Diabetes, Reports, control, SNAP, RNA Sequence Analysis, renin-binding protein, CD4, assay, CD8, Summary, Polyadenylated mRNA, Field Report, Insulin Dependent, Immune Markers, TAF1"],"additional_accession":[]},"is_claimable":false,"name":"DIABIMMUNE Transcriptomics DAC","description":"Data Access Committee EGAC00001001443","dates":{"output":"2025-1-9"},"accession":"EGAC00001001443","cross_references":{"TAXONOMY":["9606"],"pubmed":["31311800"],"EGA":["EGAS00001004071","EGAS00001004070","EGAD00001005768","EGAD00001005767"]}}