EGAGenomicsIllumina MiSeq;, HiSeq X Ten;, Illumina HiSeq 2500;https://ega-archive.org/datasets/EGAD00001001332700EGA dataset EGAD00001001332EGAThe simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.Macaulay Iain C IC, Haerty Wilfried W, Kumar Parveen P, Li Yang I YI, Hu Tim Xiaoming TX, Teng Mabel J MJ, Goolam Mubeen M, Saurat Nathalie N, Coupland Paul P, Shirley Lesley M LM, Smith Miriam M, Van der Aa Niels N, Banerjee Ruby R, Ellis Peter D PD, Quail Michael A MA, Swerdlow Harold P HP, Zernicka-Goetz Magdalena M, Livesey Frederick J FJ, Ponting Chris P CP, Voet Thierry TfalseEGAS00001001204-sc-20150415 - samplesDevelopment of a method for separation and parallel sequencing of the genomes and transcriptomes of single cells.2019-10-31 12:52:11EGAD00001001332960625915121EGAC00001000205EGAS00001001204