EGAGenomicsIllumina Genome Analyzer II;, Illumina HiSeq 2000;https://ega-archive.org/datasets/EGAD0000100139772EGA dataset EGAD00001001397EGAThe landscape of genetic alterations in lung adenocarcinoma derived from Asian patients is largely uncharacterized. Here we present an integrated genomic and transcriptomic analysis of 335 primary lung adenocarcinomas and 35 corresponding lymph node metastases from Chinese patients. Altogether 13 significantly mutated genes are identified, including the most commonly mutated gene TP53 and novel mutation targets such as RHPN2, GLI3 and MRC2. TP53 mutations are furthermore significantly enriched in tumours from patients harbouring metastases. Genes regulating cytoskeleton remodelling processes are also frequently altered, especially in metastatic samples, of which the high expression level of IQGAP3 is identified as a marker for poor prognosis. Our study represents the first large-scale sequencing effort on lung adenocarcinoma in Asian patients and provides a comprehensive mutational landscape for both primary and metastatic tumours. This may thus form a basis for personalized medical care and shed light on the molecular pathogenesis of metastatic lung adenocarcinoma.Frequent alterations in cytoskeleton remodelling genes in primary and metastatic lung adenocarcinomas.Wu Kui K, Zhang Xin X, Li Fuqiang F, Xiao Dakai D, Hou Yong Y, Zhu Shida S, Liu Dongbing D, Ye Xiaofei X, Ye Mingzhi M, Yang Jie J, Shao Libin L, Pan Hui H, Lu Na N, Yu Yuan Y, Liu Liping L, Li Jin J, Huang Liyan L, Tang Hailing H, Deng Qiuhua Q, Zheng Yue Y, Peng Lihua L, Liu Geng G, Gu Xia X, He Ping P, Gu Yingying Y, Lin Weixuan W, He Huiming H, Xie Guoyun G, Liang Han H, An Na N, Wang Hui H, Teixeira Manuel M, Vieira Joana J, Liang Wenhua W, Zhao Xin X, Peng Zhiyu Z, Mu Feng F, Zhang Xiuqing X, Xu Xun X, Yang Huanming H, Kristiansen Karsten K, Wang Jian J, Zhong Nanshan N, Wang Jun J, Pan-Hammarström Qiang Q, He Jianxing Jprojections, Forms, scale tissue, Node, Materials, rhpn2-b, AU023367, Microtrabecular, determination, Adenomas, neoplasia, Mbp1, positive regulation by symbiont of host non-apoptotic programmed cell death, LUAD., Korean American, Visible Light, Tumor, ADNOS, Tp53, Mutations, Filipino American, Cytoskeletons, parenchyma of lung, bbl, hnu, Granular Cell Adenocarcinoma, pathogenesis, Tubular Carcinoma, myd, Eastern, Microtrabecular Lattice, foton, Cribriform Carcinoma, East, Lymph, RHOBP, Eastern Asian Peoples, modification by symbiont of host biological process, Prognoses, Vietnamese American, PAPA1, Cytoplasmic Filament, stimulation by symbiont of host programmed cell death, BCC7, Adenoma, Granular Cell Carcinoma, cell process disease, tumor disease, Cytoskeletal, plant peltate hair, neoplasm (disease), Mbp-1, Tubular Carcinomas, genetic, TNFSF14, Cambodian, Microtrabecular Lattices, papilla, tumor, Hmong Americans, gamma, Tumors, Carcinoma, Radiation, pulmo, gyltl1b-b, UNQ391/PRO726, lymphatic system, familial, lamina, lung parenchyma, flanges, neoplastic disease, AI593484, Light, Adenocarcinomas, Far East, tumours, Asian unspecified, add, Cambodian Americans, adenocarcinoma of the lung, Tubular, LIGHT, Malignant Adenoma, MDDGA6, mKIAA0609, shelf, Cytoskeletal Filament, modulation by symbiont of host system process, Genetic Materials, bfy, KIAA0609, Basal Cell Adenocarcinoma, neoplasms, Genetic Material, Japanese American, East Asian Peoples, Chinese American, Carcinomas, Visible Radiations, fg, Visible Radiation, Factors, gyltl1b, HVEML, GLI3FL, adenocarcinoma of lung, AI854843, shelves, Prognostic, mdc1d, expanded, Cribriform, Filaments, non-small cell lung adenocarcinoma, INSDC_feature:gene, Pdn, projection, ridge, induction by organism of programmed cell death in other organism during symbiotic interaction, Tubular Adenocarcinomas, modification by symbiont of host morphology or physiology, nonsmall cell adenocarcinoma, Lung Adenocarcinoma, MDC1D, metastatic, light quantum, People, enr, Patient, pulmonary, enlarged, Material, Eastern Asians, AA536890, activation by symbiont of host programmed cell death, Nodes, P53, p44, bhy, Cistron, inherited genetic, PAP-A, other neoplasm, PPDIV, Prognostic Factor, bronchogenic lung adenocarcinoma, Adenocarcinoma, big, D030034H08, Lung, Lattices, Hmong American, Hmong, Cytoplasmic Filaments, lamellae, Asian, Neoplasms, regulation by symbiont of host system process, p53, peltate hair, Oxyphilic Adenocarcinoma, Gene, Prognostic Factors, 1300002E07Rik, Malignant, LFS1, Xt, process of organ, froggy, NEOPL, Gyltl1a, Granular Cell Adenocarcinomas, Ly113, P76RBE, lamella, Filament, large, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, Japanese, Cytoskeletal Filaments, Asian Indian American, Vietnamese Americans, lung, Basal Cell Adenocarcinomas, ACLS, neoplasm, Asian People, study, induction of non-apoptotic programmed cell death by other organism, American, Genetic, MDDGB6, Lichtquant, LUAD, Tubular Adenocarcinoma, Filipino, Eastern Asian People, tumour, LARGE, ridges, causes, Visible, Cribriform Carcinomas, PHS, Asians, Far East Asian, Cytoplasmic, BPFD#36, MALIGNANT AND UNSPECIFIED (INCL CYSTS AND POLYPS), Trp53, Granular Cell Carcinomas, Korean, Clients, great, causality, photon, TR2, TRP53, constitutitional genetic, Vietnamese, Chinese, laminae, Asian Indian Americans, Neoplastic Growth, Oxyphilic, PAPB, PAPA, activation by organism of non-apoptotic programmed cell death in other organism, disruption by symbiont of host cell, hemolysin activity, Basal Cell, Factor, East Asian, Cistrons, CD258, Korean Americans, Client, Xp53, neoplastic growth, Americans, GLI3-190, chemical analysis, Filipino Americans, Granular Cell, Oxyphilic Adenocarcinomas, scales, D7Ertd784e, Indian American, Asian Americans, Bph, flange, organ process, nodus lymphaticus, Lung Adenocarcinomas, Radiations, scale, Lymph Node, xgli3, HVEM-L, Asian American, Photoradiation, Cambodian American, disease of cellular proliferation, Malignant Adenomas, Chinese Americans, LTg, Japanese Americans, processes, Lymph node, East Asians, Far East Asians, lung adenocarcinoma, Photoradiations, Lattice, C85860, lymph gland, Lungs, Eastern Asian, assay, hereditary, NEOPLASMS BENIGN, Neoplasia, GCPSVasp, VASP, Data Set., DmelCG15112, ENHANCER OF ATNSI ACTIVITY, MENA, NDPP1, l(2)02029, Enb, enb, ENA, Ena, CG15112, ENA/VASPWES, Complete Transcriptome, Complete, Whole Genome, Complete Transcriptome Sequencing, Exome Sequencings, Complete Exome Sequencings, Procedures, Complete Exome, non-small cell lung carcinoma, Complete Exome Sequencing, Whole Transcriptome Sequencing, Exome, Complete Genome Sequencing, Genome Sequencing, Methodological, non-small cell carcinoma of the lung, Procedure, Methodological Study, Client, Sequencing, non-small cell lung cancer, plan specification, Study, non-small cell cancer of the lung, non-small cell carcinoma of lung, NSCLC, Exome Sequencing, method, Techniques, Whole Exome, Complete Genome, Methodological Studies, method used in an experiment., Patient, Method, Clients, Whole, Whole Exome Sequencing, Studies, Whole Transcriptome, Transcriptome Sequencing, NSCLC - non-small cell lung cancer, Non-small cell lung cancer, non-small cell cancer of lung, non-small cell lung carcinoma (disease), Transcriptome Sequencings, TechniqueAdenocarcinoma, Carcinoma, Oxyphilic, Lattices, Materials, pulmo, Microtrabecular, Adenomas, Cytoplasmic Filaments, Basal Cell, Oxyphilic Adenocarcinoma, lung parenchyma, Gene, Cistrons, Malignant, ADNOS, Adenocarcinomas, Granular Cell Adenocarcinomas, Filament, Tubular, Cytoskeletons, Malignant Adenoma, parenchyma of lung, Cytoskeletal Filaments, Granular Cell Adenocarcinoma, Cytoskeletal Filament, lung, Genetic Materials, Tubular Carcinoma, Granular Cell, Oxyphilic Adenocarcinomas, Basal Cell Adenocarcinomas, neoplasm, Basal Cell Adenocarcinoma, Microtrabecular Lattice, Genetic Material, neoplasms, Carcinomas, frequent, Genetic, Cytoplasmic Filament, Adenoma, Cytoskeletal, Granular Cell Carcinoma, Tubular Adenocarcinoma, Cribriform, Filaments, Tubular Carcinomas, Cribriform Carcinomas, Tubular Adenocarcinomas, Malignant Adenomas, Cytoplasmic, metastatic, high frequency, Cribriform Carcinoma., Granular Cell Carcinomas, pulmonary, Microtrabecular Lattices, Material, Lattice, Cistron, Lungsfalseena-DATASET-BGI-18-06-2015-04:55:13:301-17 - samplesWe sequenced 292 patients who were suffering NSCLC with Whole genome sequencing or Exome sequencing method.2017-07-26 15:39:26EGAD00001001397960626647728EGAC00001000177EGAS00001000982