EGAGenomicsIllumina HiSeq 2000;https://ega-archive.org/datasets/EGAD0000100167612EGA dataset EGAD00001001676EGAAlthough clonal selection by genetic driver aberrations in cancer is well documented, the ability of epigenetic alterations to promote tumor evolution is undefined. We used 450k arrays and next-generation sequencing to evaluate intratumor heterogeneity and evolution of DNA methylation and genetic aberrations in chronic lymphocytic leukemia (CLL). CLL cases exhibit vast interpatient differences in intratumor methylation heterogeneity, with genetically clonal cases maintaining low methylation heterogeneity and up to 10% of total CpGs in a monoallelically methylated state. Increasing methylation heterogeneity correlates with advanced genetic subclonal complexity. Selection of novel DNA methylation patterns is observed only in cases that undergo genetic evolution, and independent genetic evolution is uncommon and is restricted to low-risk alterations. These results reveal that although evolution of DNA methylation occurs in high-risk, clinically progressive cases, positive selection of novel methylation patterns entails coevolution of genetic alteration(s) in CLL.Evolution of DNA methylation is linked to genetic aberrations in chronic lymphocytic leukemia.Oakes Christopher C CC, Claus Rainer R, Gu Lei L, Assenov Yassen Y, Hüllein Jennifer J, Zucknick Manuela M, Bieg Matthias M, Brocks David D, Bogatyrova Olga O, Schmidt Christopher R CR, Rassenti Laura L, Kipps Thomas J TJ, Mertens Daniel D, Lichter Peter P, Döhner Hartmut H, Stilgenbauer Stephan S, Byrd John C JC, Zenz Thorsten T, Plass Christoph CVasp, VASP, DmelCG15112, ENHANCER OF ATNSI ACTIVITY, Data Set, Epigenetic, Epigenomic., MENA, NDPP1, l(2)02029, Enb, enb, Epigenetics, ENA, Ena, CG15112, ENA/VASPTaf[[II]]250, H2S(D2S), Taf200, dTAF[[II]]230, TAF[[II]]250, d230, dTAF[[II]]250, TAF[[II]]230, TFIID TAF250, Genomes, hydrosulfite, cel, cell, TAF200, bisulfite, l(3)84Ab, dTAFII250, Taf1p, TAF[II]250, whole genome, BG:DS00004.13, TAFII-250, CG17603, TAF250/230, TAF[[II]], EfW1, Cell, dTAF230, dTAF250, dmTAF[[II]]230, TAFII250, DmelCG17603, TAF1., Taf250, dmTAF1, SR3-5, Taf230, p230, TAF[[II]]250/230, TFIID, TAF, TAF230, TAF250Lymphoplasmacytoid Lymphomas, CLL Lymphoplasmacytoid Lymphomas, CLL, Chronic B-Cell Leukemia, Chronic B-Cell, Lymphocytic Leukemia, B-Lymphocytic Leukemias, chronic LYMPHOCYTIC, Chronic B-Lymphocytic, B-Cell, Lymphatic Leukemia, DNA Methylations, B-cell chronic lymphocytic leukaemia, B Cell, Small, B-cell chronic lymphoid leukemia, Chronic B-Cell Leukemias, Well-Differentiated Lymphocytic, Lymphoplasmacytoid Lymphoma, Well Differentiated, Chronic Lymphocytic Leukemias, Lymphomas, chronic lymphatic, Low-Grade, Lymphocytic, Leukemia, DNA methylation maintenance, B-cell chronic lymphocytic leukemia, Malignancy, Small-Cell Lymphomas, CLL Lymphoplasmacytoid Lymphoma, Plasmacytoid, Small Lymphocytic Lymphoma, Well-Differentiated Lymphocytic Lymphoma, DNA methylation, Small-Cell Lymphoma, B-Lymphocytic Leukemia, B Cell., genetic, Diffuse, Lymphoblastic Leukemias, Chronic Lymphatic Leukemias, Disrupted In B Cell Malignancy, Chronic, Diffuse Well Differentiated Lymphocytic Lymphoma, Malignancies, Chronic B-Lymphocytic Leukemias, chronic lymphatic leukaemia, Chronic Lymphocytic, constitutitional genetic, Diffuse Well-Differentiated Lymphocytic Lymphoma, Low Grade, Methylations, leukemia, B-Cell Chronic Lymphocytic Leukemia, B Cell Malignancy, Small Cell, familial, Chronic Lymphocytic Leukemia, Chronic B-Lymphocytic Leukemia, lymphoplasmacytic leukaemia, B-Cell Malignancy, Low-Grade B-Cell Malignancies, Chronic Lymphoblastic Leukemia, lymphoplasmacytic leukemia, Leukemias, chronic, B-Cell Leukemia, Well-Differentiated, Chronic Lymphatic, Small Cell Lymphoma, Lymphocytic Leukemias, B-Cell Malignancies, B Lymphocytic Leukemia, B Cell Chronic Lymphocytic Leukemia, chronic lymphatic leukemia, B Cell Leukemia, lymphocytic, Low-Grade B-Cell, Lymphoblastic Leukemia, Chronic Lymphatic Leukemia, small lymphocytic lymphoma, Lymphatic Leukemias, Lymphocytic Lymphomas, Well-Differentiated Lymphocytic Lymphomas, B-Cell Leukemias, Disrupted In B-Cell Malignancy, Lymphoblastic, Chronic Lymphoblastic, Small Lymphocytic, Chronic Lymphoblastic Leukemias, Low-Grade B-Cell Malignancy, Small-Cell, Lymphocytic Lymphoma, Lymphoplasmacytoid, CLL Lymphoplasmacytoid, Lymphoma, chronic lymphocytic leukaemia, inherited genetic, DNA, hereditary, Methylation, Small Lymphocytic LymphomasCLL, Cll, High Throughput DNA Sequencing, Nucleotide Sequencing, selection process, Massively-Parallel, Neoplasms, Benign Neoplasm, chronic LYMPHOCYTIC, High-Throughput DNA, DNA Methylations, Tumor, Malignant, Illumina, B-cell chronic lymphocytic leukaemia, Next-Generation Sequencing, cll, Relative, CML, High-Throughput RNA Sequencing, B-cell chronic lymphoid leukemia, Deep, Low, Massively Parallel Sequencing, chronic lymphatic, Deep Sequencing, me75, Ion Proton Sequencing, DNA methylation maintenance, Genetic, B-cell chronic lymphocytic leukemia, Malignancy, Illumina Sequencing, Pyrosequencing, DNA methylation, Evolution, adult chronic leukaemia, D17Mit170, Sequencing, T1, Epigenomic, Ect5, genetic, Neoplasias, Abilities, High Throughput RNA Sequencing, malignant neoplasm, adult chronic leukemia, High-Throughput, RNA Sequencing, Malignancies, chronic lymphatic leukaemia, constitutitional genetic, Methylations, Genetic Evolution, CG7937, Cancer, Tumors, High-Throughput RNA, leukemia, Malignant Neoplasm, cou, familial, Aptitudes, Chronic Lymphocytic Leukemia, lymphoplasmacytic leukaemia, High-Throughput DNA Sequencing, 311, Tl3, Tl2, lymphoplasmacytic leukemia, chronic, Ability, Ion Torrent Sequencing, Lr, MT, Benign, 93Bal, DNA Sequencing, Massively-Parallel Sequencing, Relative Risks, Neoplasm, Next-Generation, chronic lymphatic leukemia, Ion Torrent, methylation, lymphocytic, Next Generation Sequencing, Relative Risk, small lymphocytic lymphoma, primary cancer, c15, Exhibit, Molecular Evolution, Risk, Epigenetic, DmelCG7937, CG7937., Risks, Benign Neoplasms, Talents, Cancers, malignant tumor, Malignant Neoplasms, High Throughput Sequencing, Hox11-311, High-Throughput Sequencing, Talent, Bra, High Throughput Nucleotide Sequencing, chronic lymphocytic leukaemia, inherited genetic, DNA, Epigenetics, other neoplasm, hereditary, Ion Proton, Neoplasia, Methylation, High-Throughput Nucleotidefalseena-DATASET-DKFZ-Epigenomics-19-11-2015-09:41:10:900-208 - samplesTagmentation-based whole-genome bisulfite sequencing of isolated cell types from healthy controls.2017-07-26 15:39:26EGAD00001001676960624356097EGAC00001000279EGAS00001000534