EGAGenomicsN/Ahttps://ega-archive.org/datasets/EGAD0000100313610EGA dataset EGAD00001003136EGAVasp, VASP, Data Set., DmelCG15112, ENHANCER OF ATNSI ACTIVITY, MENA, NDPP1, l(2)02029, Enb, enb, ENA, Ena, CG15112, ENA/VASPH2S(D2S), matins, criteria, MHA, and leukocyte inclusions, region or site annotation, Reagent, fractured, instrument configuration, number, SCF receptor activity, and deafness, Xkl-1, Pregnancies, HiSeq 2000, gDNA, hardware, KL receptor activity, macrothrombocytopenia, Gsfsco1, EAC, PREGN, macrothrombocytopenia with dispersed leukocytic inclusions, scfr, SCO5, FTNS, SCO1, 6, Brodie Chole griffin syndrome, (2Z)-but-2-enedioate, Gsfsow3, SCFR, Gsfsco5, Reagents and Indicators, male, SOW3, average, HiSeq 3000, CYLDI, positional, Fdc, MYH9 related disorders, anatomical systems, pattern, reactivo, Genomes, 2310061K05Rik, distribution, Alport syndrome with macrothrombocytopenia, bisulfite, HardwareType, W, CYLD1, Indicators, Solution, Target Capture, geographical area, anatomical tube, MiSeq, Bs, Reagents, Sl, Methylations, reagents, Tube, proto-oncogene c-Kit, May-Hegglin thrombocytopenia, female human body, hydrosulfite, Males, USPL2, nephritis, MFT1, split, Tr-kit, female, deafness, fragmented, TUBE, PBT, MYH9 related thrombocytopenia, KIT ligand receptor activity, AI551343, BRSS, hemorrhaged, sequence, kl1-A, methylation, KIT, reactif, macrothrombocytopenia progressive deafness, dJ142L7.2, guidelines., XKrk1, Brodie Chole gryphon syndrome, tyrosine-protein kinase Kit, torn, pbt, c-KIT, bleeding disorder, positional polypeptide feature, instrument, maleate, Gestation, cracked, pooled, reagent, whole genome, kit, CDMT, CD117, MFT, HiSeq 2500, Dohle leukocyte inclusions with giant platelets, primary structure of sequence macromolecule, male human body, HiSeq 4000, sex-specific, c-kit, SBS, gender-specific anatomical structure, C-Kit, Platelet-type, Ssm, duct, macrothrombocytopenia with leukocyte inclusions, cardinality, Indicator, xkl-1, gender-specific, Females, formerly, TEM, krk1falseena-DATASET-POPS-10-01-2017-10:02:35:326-35 - samplesWe carried out whole-genome oxidative bisulfite sequencing (WGoxBS) in the placentas of two healthy female and two healthy male pregnancies generating an average genome depth of coverage of 25x. The sex-specific differential methylation pattern observed in this region was validated in additional 8 healthy placentas (including 2 from the WGoxBS) using SureSelect in-solution target capture. For WGoxBS, placental genomic DNA (4 µg) from 4 healthy pregnancies was processed to achieve 10 kb fragments with the g-Tube (Covaris), according to the manufacturer's instructions. To increase the number of uniquely sequenced reads, two independent libraries were generated for each individual. Multiplexed sequencing was carried out on the Illumina MiSeq, HiSeq 2000, and HiSeq 2500 instruments with 2x100, 2x50 and 2x125 cycles using MiSeq Reagent Kit v3, HiSeq SBS Kit v3 and HiSeq SBS Kit v4, respectively. For SureSelect in-solution capture, placental genomic DNA (3.5 µg) from 8 healthy pregnancies (including 2 from the WGoxBS) was fragmented by the Covaris S220 system according to the SureSelect Methyl-Seq target enrichment protocol (Agilent). All 8 libraries were pooled and sequenced on the Illumina HiSeq 2500 instrument with 2 × 125 cycles using HiSeq SBS Kit v4 and a single lane of the Illumina HiSeq 4000 instrument with 2 × 150 cycles using HiSeq 3000/4000 SBS Kit following Illumina's guidelines (Illumina Application Note: Epigenetics February 2016).2021-02-01 13:15:41EGAD000010031369606EGAC00001000582EGAS00001002205