EGAGenomicsIllumina HiSeq 2500;ILLUMINA, NextSeq 500;ILLUMINAhttps://ega-archive.org/datasets/EGAD00001003985442EGA dataset EGAD00001003985EGATCR- and BCR-sequencing data for The interface of malignant and immunologic clonal dynamics in high-grade serous ovarian cancerTC-NER, Ribonucleic, chemical properties, D15N2e, Mammalian, Salpinx, nested-PCR, DELTA AND GAMMA, Xkl-1, Tumor, betaTub1, AI448246, Cut, KL receptor activity, B1t, Gsfsco1, l(1)7Ba, l(1)7Bb, Methanal, Parafilm, FLASH, grade III, SCO5, 4, SCO1, 7, 9, Gsfsow3, rounded, Gsfsco5, Non Polyadenylated, RNA Gene Products, SOW3, Multicase, kus, beta1tub, mKIAA1668, CG9277, transcription-coupled NER, Formaldehyd, Oxomethane, Hemotoxylin, T, beta-particle, W, RIP25, CG11387, high grade, serous cystadenocarcinoma, Salpinges, undifferentiated, ACE, 1t, e, ch, e-, mKIAA1315, beta1Tub, scientific observation, ovarian serous adenocarcinoma, 260, Oviducts, CT, 10(6H)-pentol, Bs, serous adenocarcinoma of the ovary, Sl, xCYP26, serous adenocarcinoma of ovary, br31, Tube, BETA 56D, chemical characterization, Hydroxybrazilin, total RNA extract, ribose nucleic acid, female paramesonephric duct, transcription-coupled repair, Ct, ribonucleic acids, Tr-kit, Tubes, CED-4, grade 3, grade 4, Uterine, PBT, any method, serous carcinoma of the ovary, EST573322, AA387232, l(1)G0108, Ribonukleinsaeure, kl1-A, 11b-dihydro-, pentosenucleic acids, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, Ribonucleic acids, BcDNA:GH10590, KIT, END, fixed, PCR, grade 3/4, tyrosine-protein kinase Kit, Acid, pbt, beta-tub, 5830465M17Rik, Beta <eudicots>, Review, G3, G4, FCAALL.331, paramesonephric duct of female, INSDC_feature:gene, kit, Race, beta56D, chemical content, DmelCG9277, 2-d)pyran-3, SmokTcr, Fallopian, beta1-tub, beta(-), DTB2, beta-Tub, race, nested PCR, immunoglobulin, 6a, i6, DL4180C, Dominant negative form of Smok, T-cell receptor complex, Review of Reported Cases, RACE, beta-Tub56D, betaTub, Specbeta, beta1-Tubulin, krk1, EST820961, l(1)VE614, Mammalian Oviduct, Uterine Tube, Hematoxiline, number, e(-), SCF receptor activity, D4Ertd659e, tuba uterina, high weight, electron, l(2)br31, presence, CG5408, 4933415H02Rik, Responder protein Smok-Tcr, antibody, scfr, B-spec, cis-(+)-, Uterine Salpinx, betaspec, poorly differentiated, heavy, Gene Products, tumor sample, SCFR, anon-EST:fe3D10, grade IV, l34Eb, Fdc, betatub(56D), Academic, DmelCG8827, Formaldehyde, Mammalian Oviducts, Elektron, beta[[1]] tubulin, ANCE, Fallopian Tube, Hydroxybrasilin, beta1t, Oviduct, chemical composition., kf, study assay, Non-Polyadenylated RNA, anon-EST:fe1B3, ance, serous carcinoma of ovary, DmelCG11387, Formalin, SPEC8, AnCE, malignant ovarian serous tumour, l(2)34Eb, CG5870, fallopian tubes, salpinx, serous ovarian cancer, chemical structure, HHT1, Uterine Salpinges, measuring, beta-tubulin56D, Spec-beta, proto-oncogene c-Kit, RNA, Dmbeta1, Trb, T-lymphocyte receptor complex, ovarian serous carcinoma, XCYP26A1, Haematoxylon, Tubulin, RNS, i168, formaldehyde, count in organism, trb, 2.7.11.1, Review Literature, DmelCG5408, TCR complex, KIT ligand receptor activity, yeast nucleic acid, betaSpec, FORMALIN, serous adenocarcinoma, salpinges, b-Spec, MIRab13, Library, FORMALDEHYDE, XKrk1, T lymphocyte receptor complex, opsonin activity, ribonucleic acid, Tcr, TCR, DmelCG5870, mammalian oviduct, c-KIT, Che-1, l(1)G0074, ovary serous adenocarcinoma, l(1)G0198, NOS (morphologic abnormality), negatron, beta[[1]]-tubulin, Non Polyadenylated RNA, D15Mit260, Non-Polyadenylated, b spectrin, uterine tube (sensu Mammalia), cyp26, beta1, Benz(b)indeno(1, BG:DS08220.3, Ribonucleic Acid, CD117, serous carcinoma, Methylene oxide, beta, incised, Tub, c-kit, Oxomethylene, C-Kit, beta-spec, Uterine Tubes, Ssm, BETA, xkl-1, B cell receptor activity, Ethnicity, round, quantitative, ORW1, Spec, CG8827, presence or absence in organismVasp, VASP, DmelCG15112, ENHANCER OF ATNSI ACTIVITY, Data Set, MENA, NDPP1, l(2)02029, Enb, enb, ENA, Ena, CG15112, 2018., ENA/VASP0.00.00.00.00.0falseena-DATASET-BCCA-26-02-2018-20:04:25:014-667 - samplesEach tumor sample was cut into three pieces, yielding two end-pieces for cryovials and a middle portion placed in 10% buffered formalin. End pieces were homogenized manually and with a paddle blender (Stomacher). All paraffin-embedded blocks, including formalin-fixed tumor samples and molecular-fixed fallopian tubes, were sectioned and stained with hematoxylin and eosin prior to expert histopathological review to confirm the presence of high grade serous carcinoma. Homogenized end pieces were then flash frozen, and RNA was extracted using the miRNeasy Mini kit. Nanodrop was used to assess quality (260/280) and quantity. Total RNA samples were also QC checked using the Caliper HT RNA HiSens assay. Samples ranging from 60-255ng RNA were re-arrayed into a 96-well plate. 5'-RACE PCR was carried out as described in "The interface of malignant and immunologic clonal dynamics in high-grade serous ovarian cancer" (Zhang et al.). Briefly, this involved first round and nested PCR with TRB (TCR beta chain) and IGH (immunoglobulin heavy chain) gene-specific primers. The indexed libraries were sequenced on the Illumina HiSeq platform with paired-end 250bp reads using v2 chemistry reagents.2022-11-22 12:10:03EGAD000010039859606EGAC00000000011EGAS00001002839