EGAGenomicsIllumina HiSeq 2500;ILLUMINA, Illumina Genome Analyzer IIx;ILLUMINAhttps://ega-archive.org/datasets/EGAD0000100399264EGA dataset EGAD00001003992EGARecent studies have uncovered thousands of long non-coding RNAs (lncRNAs) in human pancreatic β cells. β cell lncRNAs are often cell type specific and exhibit dynamic regulation during differentiation or upon changing glucose concentrations. Although these features hint at a role of lncRNAs in β cell gene regulation and diabetes, the function of β cell lncRNAs remains largely unknown. In this study, we investigated the function of β cell-specific lncRNAs and transcription factors using transcript knockdowns and co-expression network analysis. This revealed lncRNAs that function in concert with transcription factors to regulate β cell-specific transcriptional networks. We further demonstrate that the lncRNA PLUTO affects local 3D chromatin structure and transcription of PDX1, encoding a key β cell transcription factor, and that both PLUTO and PDX1 are downregulated in islets from donors with type 2 diabetes or impaired glucose tolerance. These results implicate lncRNAs in the regulation of β cell-specific transcription factor networks.Human Pancreatic β Cell lncRNAs Control Cell-Specific Regulatory Networks.Akerman Ildem I, Tu Zhidong Z, Beucher Anthony A, Rolando Delphine M Y DMY, Sauty-Colace Claire C, Benazra Marion M, Nakic Nikolina N, Yang Jialiang J, Wang Huan H, Pasquali Lorenzo L, Moran Ignasi I, Garcia-Hurtado Javier J, Castro Natalia N, Gonzalez-Franco Roser R, Stewart Andrew F AF, Bonner Caroline C, Piemonti Lorenzo L, Berney Thierry T, Groop Leif L, Kerr-Conte Julie J, Pattou Francois F, Argmann Carmen C, Schadt Eric E, Ravassard Philippe P, Ferrer Jorge Jfalseena-DATASET-ICL-01-03-2018-15:32:27:049-427 - samplesWhole Human Islet paired-ended RNA-seq of 64 human pancreatic donors.2018-03-06 10:50:59EGAD00001003992960628041957EGAC00001000853EGAS00001002865