{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"dataset_type":["Illumina HiSeq 2500;ILLUMINA"],"full_dataset_link":["https://ega-archive.org/datasets/EGAD00001004047"],"sample_count":["32"],"description":["EGA dataset EGAD00001004047"],"repository":["EGA"],"title":["Total RNA sequencing of 32 chronic lymphocytic leukemia (CLL) patients"],"name_synonyms":["Vasp, VASP, DmelCG15112, ENHANCER OF ATNSI ACTIVITY, Data Set, MENA, NDPP1, l(2)02029, Enb, enb, ENA, Ena, CG15112, 2018., ENA/VASP"],"description_synonyms":["Lymphocyte antigen T1|Leu-1, CLL, Cll, B Cells, Ly-A, GRP1/cytohesin 1, Balearic Islands, Bursa-Dependent Lymphocytes, Reagent, Blood, instrument configuration, B cell, number, chronic LYMPHOCYTIC, SCF receptor activity, Xkl-1, B-cell chronic lymphocytic leukaemia, hardware, CG11633, cll, Fluorescence-Activated, cytohesin/GRP1, CML, KL receptor activity, Fluorescence-Activated Cell Sortings, Microfluorometry, Gsfsco1, Ly-12, scfr, isolation, B-cell chronic lymphoid leukemia, Lyt-1, PBMC, GRP1, Grp1, SCO5, B4, SCO1, Ly-1, Flow Microfluorimetry, Gsfsow3, Cell Sortings, SCFR, Gsfsco5, lymphocyte antigen 1, Reagents and Indicators, chronic lymphatic, SOW3, Cytofluorometry, Fdc, B-cell, anatomical systems, B-cell chronic lymphocytic leukemia, reactivo, Fluorescence Activated Cell Sorting, Flow Cytofluorometries, Microfluorometries, PTPSTEP, l(2)SH2 0323, HardwareType, Sciences, CD19, purification, W, adult chronic leukaemia, T1, Indicators, Ect5, isolation and purification, Neural-specific protein-tyrosine phosphatase, B lymphocyte, HHT1., Clients, l(2)k08110, Flow, adult chronic leukemia, Flow Microfluorometries, Cytometry, Bs, chronic lymphatic leukaemia, Reagents, Sl, Flow Cytofluorometry, HHT1, CG7937, reagents, Cytometries, Sortings, GPH, leukemia, Cell Sorting, proto-oncogene c-Kit, Fluorescence-Activated Cell Sorting, bead, whole blood, total RNA extract, Edg, B Lymphocytes, PBMC cell, Chronic Lymphocytic Leukemia, lymphoplasmacytic leukaemia, Canary Islands, 311, Tr-kit, stepk, LEU1, Flow Cytometries, lymphoplasmacytic leukemia, Client, Cell, chronic, AW495831, Microfluorimetry, whole transcriptome, PBT, 93Bal, KIT ligand receptor activity, B-lymphocyte surface antigen B4, 3.1.3.48, kl1-A, lymphocyte antigen T1/Leu-1, chronic lymphatic leukemia, median, lymphocytic, KIT, Flow Microfluorometry, reactif, l(2)SH0323, END, Library, XKrk1, CYH1, tyrosine-protein kinase Kit, small lymphocytic lymphoma, ly-1, pbt, c-KIT, Cytofluorometries, c15, Peripheral Blood, PBM cell, T-cell surface antigen Leu-12, instrument, Step, CG11628, DmelCG7937, Magnetic, Bursa-Equivalent Lymphocyte, reagent, Striatum-enriched protein-tyrosine phosphatase, kit, CD117, Fluorescence-Activated Cell, HiSeq 2500, DmelCG11628, Reticuloendothelial System, Hox11-311, c-kit, Patient, C-Kit, B-Lymphocyte, STEP, differentiation antigen CD19, Ssm, PBMCs, Indicator, cardinality, Differentiation antigen CD19, Sorting, xkl-1, chronic lymphocytic leukaemia, CD5, B-lymphocyte, ORW1, CVID3, peripheral blood mononuclear cell, krk1"],"additional_accession":[]},"is_claimable":false,"name":"ena-DATASET-ULB-19-03-2018-10:39:06:920-313 - samples","description":"Peripheral blood mononuclear cells (PBMC) of CLL patients were isolated by density-gradient centrifugation over Linfosep (Biomedics, Madrid, Spain). B cells were purified with a CD19+ magnetic-bead system (MidiMACS, Miltenyi Biotec, Bergish Gladbash, Germany) according to the manufacturers’ instructions. Mean B-cell purity was >99% and the mean percentage of CD5+/CD19+ cells after purification was >98%, as measured by flow cytometry. Total RNA was extracted from purified cells in a single step using TriPure Isolation Reagent (Roche Applied Science, Vilvoorde, Belgium).Whole transcriptome sequencing libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina). Libraries underwent 2 × 76 bp paired-end sequencing on a HiSeq 2500 instrument (Illumina). The median number of paired-end reads was 60.5 million (range, 49.7-79.7 million).","dates":{"updated":"2020-07-16 15:33:08"},"accession":"EGAD00001004047","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAC00001000866","EGAS00001002894"]}}