EGAGenomicsN/Ahttps://ega-archive.org/datasets/EGAD0000100518154EGA dataset EGAD00001005181EGAWe performed genomic and transcriptomic sequencing of 133 combined hepatocellular and intrahepatic cholangiocarcinoma (cHCC-ICC) cases, including separate, combined, and mixed subtypes. Integrative comparison of cHCC-ICC with hepatocellular carcinoma and intrahepatic cholangiocarcinoma revealed that combined and mixed type cHCC-ICCs are distinct subtypes with different clinical and molecular features. Integrating laser microdissection, cancer cell fraction analysis, and single nucleus sequencing, we revealed both mono- and multiclonal origins in the separate type cHCC-ICCs, whereas combined and mixed type cHCC-ICCs were all monoclonal origin. Notably, cHCC-ICCs showed significantly higher expression of Nestin, suggesting Nestin may serve as a biomarker for diagnosing cHCC-ICC. Our results provide important biological and clinical insights into cHCC-ICC.Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes.Xue Ruidong R, Chen Lu L, Zhang Chong C, Fujita Masashi M, Li Ruoyan R, Yan Shu-Mei SM, Ong Choon Kiat CK, Liao Xiwen X, Gao Qiang Q, Sasagawa Shota S, Li Yanmeng Y, Wang Jincheng J, Guo Hua H, Huang Qi-Tao QT, Zhong Qian Q, Tan Jing J, Qi Lisha L, Gong Wenchen W, Hong Zhixian Z, Li Meng M, Zhao Jingmin J, Peng Tao T, Lu Yinying Y, Lim Kiat Hon Tony KHT, Boot Arnoud A, Ono Atushi A, Chayama Kazuaki K, Zhang Zemin Z, Rozen Steve George SG, Teh Bin Tean BT, Wang Xin Wei XW, Nakagawa Hidewaki H, Zeng Mu-Sheng MS, Bai Fan F, Zhang Ning Nfalseeaa4975f-4514-44a7-baa4-9404c42156a2 - samplesWhole genome sequencing dataset of 54 tumor/normal samples of 25 cHCC-ICC cases.2019-09-19 10:54:21EGAD00001005181960631130341EGAC00001001273EGAS00001003093