{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"dataset_type":["N/A"],"full_dataset_link":["https://ega-archive.org/datasets/EGAD00001007032"],"sample_count":["240"],"description":["EGA dataset EGAD00001007032"],"repository":["EGA"],"title":["Whole-genome sequences of single-cell derived clonal samples and bulk blood samples from human"],"additional_accession":[]},"is_claimable":false,"name":"5e853f8e-8a76-4e4a-bb83-33ce4061654d - samples","description":"Human single cells were clonally expanded by culture and whole-genome sequenced. This dataset includes 334 clonal samples and 7 blood bulks from seven individuals (DB2, DB3, DB5, DB6, DB8, DB9, DB10). We extracted genomic DNA materials from clonally expanded cells and matched peripheral blood using DNeasy Blood and Tissue kits (Qiagen) according to the protocol. DNA libraries for WGS were generated by an Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) from 1 Ã‚Âµg of genomic DNA materials. WGS was performed on either the Illumina HiSeq X platform or the NovaSeq 6000 platform to generate mean coverage of 25.2X for 374 clonally expanded cells and 94.8X for 7 matched blood tissues.","dates":{"updated":"2021-06-21 12:24:58"},"accession":"EGAD00001007032","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAC00001000587","EGAS00001004824"]}}