{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"dataset_type":["N/A"],"full_dataset_link":["https://ega-archive.org/datasets/EGAD00001007764"],"sample_count":["988"],"description":["EGA dataset EGAD00001007764"],"repository":["EGA"],"title":["1M-scBloodNL"],"additional_accession":[]},"is_claimable":false,"name":"c77ececa-f591-44ac-b76b-aa8458182f09 - samples","description":"Here we present the 1M-scBloodNL study, in which we performed single-cell RNA-seq on 120 individuals of the Northern Netherlands population cohort Lifelines. For each individual peripheral blood mononuclear cells (PBMCs) were sequenced in an unstimulated condition, and after 3 and 24 hour in vitro stimulation with C. albicans (CA), M. tuberculosis (MTB) and P. aeruginosa (PA), totalling approximately 1.3 million cells. scRNA-seq was conducted with the 10X Genomics 3'-end v2 (72 libraries) and v3 (33 libraries) technology. In general, each library contains PBMCs from 8 donors and 2 different stimulation-timepoint combinations. Donors were demultiplexed using a combination of SoupOrCell (https://www.nature.com/articles/s41592-020-0820-1) and genotype information to assign the correct donor to a donor-specific cell cluster.","dates":{"updated":"2021-06-30 15:23:41"},"accession":"EGAD00001007764","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAC00001002165","EGAS00001005376"]}}