Single-cell multi-omics of human clonal hematopoiesis reveals that DNMT3A R882 mutations perturb early progenitor states through selective hypomethylation.

EGAGenomicsN/Ahttps://ega-archive.org/datasets/EGAD000010089855EGA dataset EGAD00001008985EGASingle-cell multi-omics of human clonal hematopoiesis reveals that DNMT3A R882 mutations perturb early progenitor states through selective hypomethylation.falsec259e00e-d362-45f3-b447-6386066379f9 - samplesWe profiled CD34+ enriched cells from GCSF mobilized bone marrow samples (n = 4) using single-cell RNA sequencing (10X) with targeted genotyping, and single-cell DNA methylation (RRBS) with single-cell RNA sequencing (Smart-Seq2) and targeted genotyping. A 5th CH bone marrow aspirate sample was obtained to validate observed results.2022-06-27 16:46:05EGAD000010089859606EGAC00001002735EGAS00001006364