{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"dataset_type":["N/A"],"full_dataset_link":["https://ega-archive.org/datasets/EGAD00001009686"],"sample_count":["64"],"description":["EGA dataset EGAD00001009686"],"repository":["EGA"],"title":["Postmortem Single Nuclei and Bulk RNA-seq data of the Motor Cortex and Spinal Cord for Healthy, C9ALS and sALS Patients"],"additional_accession":[]},"is_claimable":false,"name":"b6359283-0636-4e50-a9e7-160863c7e808 - samples","description":"This dataset contains both snRNA-seq and bulk RNA-seq data from 19 different patients, comprising of 9 healthy controls (4 Spinal Cord, 5 Motor Cortex) as well as 5 C9ALS patients and 5 sporadic ALS patients, each with paired data from the spinal cord and motor cortex. For the snRNA-seq data, fastq files containing the raw reads are provided, many of these samples were pooled for sequencing and subsequently require demultiplexing using SNPs using a tool such as freemuxlet. The paired bulk RNA-seq data (raw fastq files) can be used to acquire the ground truth per patient - a list of pooled samples can be found in map.txt. snRNA-seq data was produced using the 10X Genomics 3' v3 kit and bulk RNA-seq was produced using the Illumina TruSeq v2 kit.","dates":{"updated":"2022-11-04 21:35:51"},"accession":"EGAD00001009686","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAC00001002939","EGAS00001006711"]}}