<HashMap><database>EGA</database><scores/><additional><omics_type>Genomics</omics_type><dataset_type>N/A</dataset_type><full_dataset_link>https://ega-archive.org/datasets/EGAD00001009686</full_dataset_link><sample_count>64</sample_count><description>EGA dataset EGAD00001009686</description><repository>EGA</repository><title>Postmortem Single Nuclei and Bulk RNA-seq data of the Motor Cortex and Spinal Cord for Healthy, C9ALS and sALS Patients</title></additional><is_claimable>false</is_claimable><name>b6359283-0636-4e50-a9e7-160863c7e808 - samples</name><description>This dataset contains both snRNA-seq and bulk RNA-seq data from 19 different patients, comprising of 9 healthy controls (4 Spinal Cord, 5 Motor Cortex) as well as 5 C9ALS patients and 5 sporadic ALS patients, each with paired data from the spinal cord and motor cortex. For the snRNA-seq data, fastq files containing the raw reads are provided, many of these samples were pooled for sequencing and subsequently require demultiplexing using SNPs using a tool such as freemuxlet. The paired bulk RNA-seq data (raw fastq files) can be used to acquire the ground truth per patient - a list of pooled samples can be found in map.txt. snRNA-seq data was produced using the 10X Genomics 3' v3 kit and bulk RNA-seq was produced using the Illumina TruSeq v2 kit.</description><dates><updated>2022-11-04 21:35:51</updated></dates><accession>EGAD00001009686</accession><cross_references><TAXONOMY>9606</TAXONOMY><EGA>EGAC00001002939</EGA><EGA>EGAS00001006711</EGA></cross_references></HashMap>