{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"dataset_type":["N/A"],"full_dataset_link":["https://ega-archive.org/datasets/EGAD00001010837"],"sample_count":["1"],"description":["EGA dataset EGAD00001010837"],"repository":["EGA"],"title":["Transcriptomic analyses of a large cohort of adult B cell acute lyphoblastic leukemia"],"additional_accession":[]},"is_claimable":false,"name":"b598b206-a3c4-4aa6-a002-ff79cf6ad920 - samples","description":"Total mononuclear cells (MNC) were isolated from peripheral blood (PB) or bone marrow (BM) samples of 105 Ph-/-/- (B-cell acute lymphoblastic leukemia [B-ALL] triple negative) and 31 Ph+ B-ALL adult patients using Lymphosep (Biowest, Nuaillé, France). A total of 15 samples from healthy subjects were processed including hematopoietic stem-progenitor cells (CD34+) from bone marrow specimens (n = 3), and bone marrow mononuclear cell samples (n = 3) from STEMCELL Technologies (Vancouver, Canada). PB MNC samples (n=5) and cord blood samples (n = 4). CD34+ cells were enriched from cord blood samples by immunomagnetic separation (CD34 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany).Libraries were prepared using the TruSight RNA Pan-Cancer Panel Kit (Illumina, San Diego, California, USA), following the manufacturer’s protocol. Sequencing was performed using the Illumina MiSeq instrument.","dates":{"updated":"2023-05-10 10:45:37"},"accession":"EGAD00001010837","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAC00001003223","EGAS00001007217"]}}