Healthy human B-lymphopoiesis RNA-Seq reference using FACS sorted bone marrow aspirates

EGAGenomicsN/Ahttps://ega-archive.org/datasets/EGAD0000101091728EGA dataset EGAD00001010917EGAHealthy human B-lymphopoiesis RNA-Seq reference using FACS sorted bone marrow aspiratesfalse29c4fa94-257b-4be1-9238-7c98772f3b1a - samplesTo define a transcriptomic reference of human B lymphopoiesis, bone marrow aspirates were obtained from n=4 healthy donors (study registration DRKS00023583). After immunodensity cell separation, samples were FACS-sorted into 7 established lymphopoietic differentiation stages. RNA was extracted from 5,000-320,000 cells per differentiation stage and subjected to ultra-low-input RNA sequencing after generation of stranded sequencing libraries.2023-06-21 14:49:26EGAD000010109179606EGAC00001003270EGAS00001007305