{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"dataset_type":["N/A"],"full_dataset_link":["https://ega-archive.org/datasets/EGAD00001012437"],"sample_count":["32"],"description":["EGA dataset EGAD00001012437"],"repository":["EGA"],"title":["RNA sequencing of in vitro generated suppressive myeloid cells using parental and Sialidase expressing A549 cancer cell lines"],"additional_accession":[]},"is_claimable":false,"name":"3cd56ac5-0953-459f-9c48-4a4f99c234cd - samples","description":"Fresh peripheral blood mononuclear cells of four human donors were cultured together with either lung adenocarcinoma A549 cancer cells or A549-expressing H1N1 Sialidase cancer cells. These treatments induced the differentiation of donor cells into immunosuppressive MDSC-like cells, which were further subjected to bulk RNA sequencing. \n\nRNA-seq TruSeq libraries were generated from polyA-enriched mRNA isolated from the samples, and sequenced in paired-end mode on 4 lanes of an Illumina NextSeq 500 flow-cell","dates":{"updated":"2024-03-22 15:56:19"},"accession":"EGAD00001012437","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAC00001002981","EGAS00001007618"]}}