EGAGenomicsN/Ahttps://ega-archive.org/datasets/EGAD0000101243732EGA dataset EGAD00001012437EGARNA sequencing of in vitro generated suppressive myeloid cells using parental and Sialidase expressing A549 cancer cell linesfalse3cd56ac5-0953-459f-9c48-4a4f99c234cd - samplesFresh peripheral blood mononuclear cells of four human donors were cultured together with either lung adenocarcinoma A549 cancer cells or A549-expressing H1N1 Sialidase cancer cells. These treatments induced the differentiation of donor cells into immunosuppressive MDSC-like cells, which were further subjected to bulk RNA sequencing.
RNA-seq TruSeq libraries were generated from polyA-enriched mRNA isolated from the samples, and sequenced in paired-end mode on 4 lanes of an Illumina NextSeq 500 flow-cell2024-03-22 15:56:19EGAD000010124379606EGAC00001002981EGAS00001007618