EGAGenomicsIllumina HiSeq 2000Epigeneticshttps://ega-archive.org/studies/EGAS00001000414EGAEGA study EGAS00001000414Methylation analysis in GM01240 and GM01247EGArestrictedassay, GM01247., methylation, determination, Methylations, GM01240, chemical analysisprojections, Co-Immunoprecipitations, H2S(D2S), other disease, scale tissue, human being, Respect, Materials, DNS, single-organism developmental process, Procedures, methionine aminopeptidase activity, Methylated DNA Immunoprecipitation sequencing, (Deoxyribonucleotide)n, lamellae, postnatal development, peltate hair, Gene, growth and development, DNA Methylations, broad, process of organ, Deoxyribonucleic acids, element, Human, Co Immunoprecipitation, lamella, Techniques, method, Personal, Caucasian, Homo sapiens, Deoxyribonucleic Acid, diseases, Method, method used in an experiment, Island, Studies, Immune Precipitations, Line, disease or disorder, 2, diseases and disorders, Man, Technique, familial adenomatous polyposis, Immune Precipitation, MAP, peptidase M activity, adenomas, study, rabGAPLP, CpG Cluster, L-methionine aminopeptidase activity, thymus nucleic acid, human disease, DNA methylation maintenance, Occidental, Man (Taxonomy), Genetic, Genomes, familial adenomatous polyposis 2, plant peltate hair, Co-Immunoprecipitation, Precipitation, study., RabGAP-5, bisulfite, Double Stranded, Deoxyribonucleic acid, procedures, ridges, DNA methylation, CpG, Epigenomic, autosomal recessive multiple colorectal adenomas, non-neoplastic, Study, CpG Clusters, Immune, Human Genomes, Methodological Studies, RUSC3, papilla, Personal Respect, disorder, Homo sapiens disease, Double-Stranded DNA, (Deoxyribonucleotide)m, deoxyribonucleic acids, Precipitations, DNAn, elements, laminae, Methylations, atom, CpG-Rich Islands, MUTYH-Associated Polyposis, wide/broad, hydrosulfite, DNAn+1, Modern, MYH-associated polyposis, lamina, disorders, autosomal recessive familial adenomatous polyposis, Cell Lines, Maps, flanges, white, GM01247, medical condition, Double-Stranded, Clusters, Procedure, Cistrons, GM01240, Cell, results, (Deoxyribonucleotide)n+m, development, Human Genome, European, shelf, Diseases, Genetic Materials, condition, atomo, atomus, techniques, scales, ds-DNA, desoxyribose nucleic acid, atome, Caucasoid, Genetic Material, flange, organ process, autosomal recessive, Lines, Caucasians, RUTBC3, CpG Island, Element, scale, Epigenetic, shelves, atoms, postnatal growth, Dignity, Methodological, whole genome, MYH-Associated Polyposis, Methodological Study, projection, ridge, human, Islands, CpG-Rich Island, RABGAP5, plan specification, CpG Rich Islands, processes, disease, wide, Cluster, Whites, Material, Modern Man, multiple colorectal, ds DNA, Desoxyribonukleinsaeure, microarray, MAP syndrome, White, Cistron, DNA, Epigenetics, growth, Methylation, FAP2, methodology, CpG-Rich, colorectal adenomatous polyposisfalseMethylation analysis in GM01240 GM01247DNA methylation is one of the most studied epigenetic marks in the human genome, with the result that the desire to map the human methylome has driven the development of several methods to map DNA methylation on a genomic scale. Our study presents the first comparison of two of these techniques - the targeted approach of the Infinium HumanMethylation450 BeadChip� with the immunoprecipitation and sequencing-based method, MeDIP-seq. Both methods were initially validated with respect to bisulfite sequencing as the gold standard and then assessed in terms of coverage, resolution and accuracy. The two methods were compared using DNA from two cell lines: GM01240 (XX) and GM01247 (XY), a sibling pair of European descent. The regions of the methylome that can be assayed by both methods and those that can only be assayed by one method were determined and the discovery of differentially methylated regions (DMRs) by both techniques was examined. Our results show that the Infinium HumanMethylation450 BeadChip� and MeDIP-seq show a good positive correlation (Spearman correlation of 0.68) on a genome-wide scale and can both be used successfully to determine differentially methylated loci in RefSeq genes, CpG islands, shores and shelves. MeDIP-seq however, allows a wider interrogation of methylated regions of the human genome, including thousands of non-RefSeq genes and repetitive elements, all of which may be of importance in disease. In our study MeDIP-seq allowed the detection of 15,709 differentially methylated regions, nearly twice as many as the array-based method (8070), which may result in a more comprehensive study of the methylome.2020-09-22 11:25:40EGAS000010004149606EGAD00001000326EGAC00001000051